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R&D Systems™ Recombinant Human KGF/FGF-7 GMP Protein, CF

Product Code. p-200112652
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Quantity:
1 mg
50 μg
Unit Size:
1mg
50µg
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Product Code. 30306760

Brand: R&D Systems™ BTKGFGMP050

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Description

Recombinant Protein

Measured in a cell proliferation assay using Ba/F3 mouse pro B cells transfected with human FGF RIIb. The ED50 for this effect is 6.00-60.0 ng/mL. The specific activity of recombinant human KGF/FGF-7 is >8.16 x 105 units/mg, which is calibrated against the human KGF/FGF-7 WHO Standard (NIBSC code: 03/150).
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P21781.1
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and EDTA with Trehalose.
Gene ID (Entrez) 2252
Molecular Weight (g/mol) MolecularWeight-observed: 20 kDa, under reducing conditions., MolecularWeight-theroretical: 19 kDa
Quantity 50 μg
Source E. coli-derived human KGF/FGF-7 protein Cys32 - Thr194, with an N-terminal Met Produced using non-animal reagents in an animal-free laboratory. Manufactured and tested under cGMP guidelines.
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU per 1 μg of the protein by the LAL method.
Gene Alias FGF7, FGF-7, fibroblast growth factor 7HBGF-7, HBGF7, HBGF-7, Heparin-binding growth factor 7, keratinocyte growth factor, KGFfibroblast growth factor 7 (keratinocyte growth factor)
Conjugate Unconjugated
Reconstitution Reconstitute at 500 μg/mL in sterile water.
Purity or Quality Grade >97%, by SDS-PAGE with quantitative densitometry by Coomassie Blue Staining. The molecular weight by mass spectrometry is 18927 Da ±61 Da
Protein KGF/FGF-7
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