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  6. Invitrogen™ Rat anti-Mouse IgG1 Secondary Antibody, APC, eBioscience™

Invitrogen™ Rat anti-Mouse IgG1 Secondary Antibody, APC, eBioscience™

Product Code. 13400169
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Quantity:
100 μg
25 μg
Unit Size:
100µg
25mg
Product Code. Quantity unitSize
13400169 25 μg 25mg
15578086 100 μg 100µg
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Product Code. 13400169

Brand: Invitrogen™ 17401580

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This item is not returnable. View return policy

Description

Rat Monoclonal Secondary Antibody

Description: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies. Applications Reported: This M1-14D12 antibody has been reported for use in flow cytometric analysis. Applications Tested: This M1-14D12 antibody has been tested by flow cytometric analysis of cells stained with a mouse IgG1 primary antibody. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Mouse IgG1
Applications Flow Cytometry, Immunohistochemistry (Frozen)
Classification Monoclonal
Clone M1-14D12
Concentration 0.2 mg/mL
Conjugate APC
Formulation PBS with 0.09% sodium azide; pH 7.2
Host Species Rat
Purification Method Affinity chromatography
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Mouse
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Secondary Antibody
Form Liquid
Isotype IgG
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