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Description
Thermo Scientific™ Unstained Protein Molecular Weight Marker is a mixture of seven native proteins (14.4kDa to 116kDa) for use as size standards in protein electrophoresis (SDS-PAGE).
Highlights:
Size range – seven proteins spanning 14.4 to 116kDa
Ready-to-use – supplied in a loading buffer for direct loading on gels
Sharp bands – equal intensity upon coomassie or silver staining
Quality tested – each lot evaluated by SDS-PAGE and Western blotting
Includes:
Proteins (0.1 to 0.2mg/mL of each) in 62.5mM Tris-HCl (pH 7.0 at 25°C), 1mM EDTA, 2% SDS, 50mM DTT, 30mM NaCl, 1mM NaN3, 0.01% bromophenol blue and 50% glycerol; Component proteins: beta-galactosidase (116kDa), bovine serum albumin (66.2kDa), ovalbumin (45.0kDa), lactate dehydrogenase (35.0kDa), REase Bsp98I (25.0kDa), beta-lactoglobulin (18.4kDa), lysozyme (14.4kDa)
Recommended for:
Accurate protein sizing on SDS-polyacrylamide gels and Western blots
Specifications
Specifications
| Content And Storage | Contents: two vials of 1 mL each Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 50 mM DTT, 30 mM NaCl, 0.01% bromophenol blue and 50% glycerol Storage: Upon receipt store at -20°C |
| Number of Markers | 7 |
| Ready to Load | Yes |
| Size Range | 14.4 to 116 kDa |
| Gel Compatibility | Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels |
| Molecular Weight (g/mol) | 116, 66.2, 45, 35, 25, 18.4, 14.4 kDa |
| Quantity | 2 x 1 mL |
| Shipping Condition | Approved for shipment on Wet or Dry Ice |
| Product Line | Pierce |
| Product Type | Protein Ladder |
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For Research Use Only. Not for use in diagnostic procedures.
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