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  5. Thermo Scientific™ Pierce™ Fast Western Blot Kit, ECL Substrate

Thermo Scientific™ Pierce™ Fast Western Blot Kit, ECL Substrate

Product Code. 10510024 Shop All Thermo Scientific Products
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Quantity:
1 L Kit
200 mL Kit
Unit Size:
1 set
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Product Code. 10510024

Brand: Thermo Scientific™ 35050

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This item is not returnable. View return policy

Description

Optimized reagents that shorten the time to perform a typical Western blot from 4 hours to approximately 55 minutes.

Thermo Scientific™ Pierce Fast Western Blot Kit, ECL Substrate contains optimized reagents that shorten the time to perform a typical Western blot from 4 hours down to approximately 55 minutes.

The kit provides all the reagents necessary to complete a Western blot being probed with a mouse or rabbit primary antibody. The protocol requires minimal hands-on time and yields results comparable to classic Western blotting with ECL. The included Pierce ECL Substrate produces a chemiluminescent signal, which is detected using photographic or other imaging methods. Blots can be repeatedly exposed to film to obtain optimal results or stripped of the primary antibody and immunodetection reagents and reprobed.

Highlights:

  • Fast – all the sensitivity of Pierce ECL substrate; saves three to four hours over traditional blotting methods

  • Convenient – no expensive hardware or extra bench space needed; eliminates membrane clogging issues

  • Economical – costs about the same as a classical Western blot; no expensive consumables or extra equipment

  • Easy – optimized kits includes all reagents except primary antibody

  • Versatile – kits work with both rabbit and mouse primary antibodies

Includes:

Kits contain all reagents to perform Western blots probed with mouse and rabbit primary antibodies, including: Fast Western Antibody Diluent; Fast Western 10X Wash Buffer; Fast Western Optimized HRP Reagent; Pierce ECL Detection Reagent 1; Pierce ECL Detection Reagent 2

TRUSTED_SUSTAINABILITY

Specifications

Product Type Western Blot Kit
Detection Method Chemiluminescence
Form Liquid
For Use With (Application) Western Blot
Membrane Compatibility Nitrocellulose, PVDF
Product Line Pierce™
Quantity 1 L Kit
Conjugate HRP
Substrate Horseradish Peroxidase
Reactivity Mouse, Rabbit
Sufficient For 25 Mini Blots
Content And Storage Sufficient For: 25 mini blots probed with mouse or rabbit primary antibodies
• Fast Western Antibody Diluent, 500 mL
• Fast Western 10X Wash Buffer, 250 mL
• Fast Western Optimized HRP Reagent, 25 mL
• Pierce ECL Detection Reagent 1, 125 mL
• Pierce ECL Detection Reagent 2, 125 mL

Store at 4°C.
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Safety and Handling

compliance-icons
Product Identifier
  • Fast Western Blot Kit, ECL Substrate
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Skin sensitiser Category 1
 Hazard Statement
  • H315-Causes skin irritation.
  • H317-May cause an allergic skin reaction.
  • H319-Causes serious eye irritation.
 Precautionary Statement
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P272-Contaminated work clothing should not be allowed out of the workplace.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P332+P313-If skin irritation occurs: Get medical advice/attention.
  • P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
  • P362+P364-Take off contaminated clothing and wash it before reuse.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : Sodium chloride, Tris (hydroxymethyl) aminomethane, Nonidet

For Research Use Only. Not for use in diagnostic procedures.

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