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p73 Antibody (5B429) - BSA Free, Novus Biologicals™

Product Code. 18082734 Shop All Bio Techne Products
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Quantity:
0.025 mg
Unit Size:
0.1mg
25µg
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18082734 0.025 mg 25µg
18798523 - 0.1mg
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Product Code. 18082734 Supplier Novus Biologicals Supplier No. NBP224737SS

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Mouse Monoclonal Antibody has been used in 56 publications

p73 Monoclonal specifically detects p73 in Human, Mouse, Rat samples. It is validated for Western Blot, Simple Western, Chromatin Immunoprecipitation, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Intracellular), CyTOF-ready.
TRUSTED_SUSTAINABILITY

Specifications

Antigen p73
Applications Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Classification Monoclonal
Clone 5B429
Concentration 1.0 mg/mL
Conjugate Unconjugated
Dilution Western Blot 2 ug/ml, Simple Western 1:25, Flow Cytometry 1 ug/mL. Use reported in scientific literature (PMID 21152443), Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 5-15 ug/ml, Immunohistochemistry-Frozen 5-15 ug/ml, Flow (Intracellular), Chromatin Immunoprecipitation (ChIP) 1:20-1:1000, CyTOF-ready
Gene Accession No. O15350
Gene Alias p53-related protein, P73p53-like transcription factor, TA p73, TAp73, tumor protein p73
Gene Symbols TP73
Host Species Mouse
Immunogen This antibody was raised against full-length human p73. It reacts with an epitope that is located on the N-terminal region of human p73. It does not cross react with p53.
Purification Method Protein G purified
Quantity 0.025 mg
Regulatory Status RUO
Research Discipline Apoptosis, Cancer, Tumor Suppressors
Primary or Secondary Primary
Gene ID (Entrez) 7161
Test Specificity p73 antibody (clone 5B429) recognizes all of the TAp73 isoforms i.e. isoforms with an intact NH2-terminal transactivation domain (Thottassery et el, 2006, PMID 16505115). Sayan et al 2005 (PMID 15781249) showed that p73 IMG-246/ NBP2-24737 reacted with all tested TAp73 isoforms but not deltaNp73 whereas p73 antibody clone 5B1288 (NB100-56674/ IMG-259A ) showed reactivity against all p73 isoforms including delta Np73. Caspase-cleaved p73 fragments, including 55 kDa and 45 kDa, have been described in literature (Sayan et al, 2008).
Target Species Human, Mouse, Rat
Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Form Purified
Isotype IgG1 κ
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