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Lonza™ P3 Primary Cell 96-well Nucleofector™ Kit

Product Code. 11506539
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Quantity:
2.25 mL P3 Primary Cell Nucleofector™ Solution, 0.5 mL Supplement 1, 50 μg pmax GFP™ Vector (1 μg/μL in 10 mM Tris pH 8.0), 1 x 96-well Nucleocuvette™ Plate (20 μL)
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11506539 2.25 mL P3 Primary Cell Nucleofector™ Solution, 0.5 mL Supplement 1, 50 μg pmax GFP™ Vector (1 μg/μL in 10 mM Tris pH 8.0), 1 x 96-well Nucleocuvette™ Plate (20 μL) Each
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Product Code. 11506539 Supplier Lonza™ Supplier No. V4SP3096

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Description

For medium-throughput transfection of primary cells, e.g., human T cells, CD34 or rat neurons, when working with the 96-well Shuttle™ Add-On.

  • High transfection efficiencies for primary cells.
  • Compatible with liquid handling systems for automated high-throughput electroporation.
  • Conditions are transferable between 4D-Nucleofector™ System, 96-well Shuttle™ System and 384-well Nucleofector™ System.
  • Applications:
    • Transfection of low cell numbers in the range of 5 x 104 to 1 x 106 cells.
    • High-throughput applications, e.g., screens of arrayed cDNA, RNAi or CRISPR libraries.
  • This kit has been determined to be optimal for transfecting following cell types:
    • B cell (human)
    • CD34+ cell (human)
    • Chondrocyte (human)
    • DC (human)
    • DC (mouse), mature
    • ES (human), H9
    • ES (mouse)
    • Hepatocyte (human)
    • HMEC (human)
    • Keratinocyte (human)
    • Macrophage (human)
    • Monocyte (human)
    • Neuron (rat), brain Hi/Cx
    • NHBE (human)
    • RPE (human)
    • T cell (human), stim.
    • T cell (mouse), unstim..
TRUSTED_SUSTAINABILITY

Specifications

Color Clear
Vector pmaxGFP™
For Use With (Equipment) 96-well Shuttle™ Add-On
For Use With (Application) Medium-throughput primary cell transfection
Includes P3 Primary Cell Nucleofector™ Solution, Supplement 1, pmaxGFP™ Vector (1μg/μL in 10mM Tris, pH 8.0; 50 μg), 96-well Nucleocuvette™ Plate
No. of Reactions 96
pH 7.1 to 7.3
Quantity 2.25 mL P3 Primary Cell Nucleofector™ Solution, 0.5 mL Supplement 1, 50 μg pmax GFP™ Vector (1 μg/μL in 10 mM Tris pH 8.0), 1 x 96-well Nucleocuvette™ Plate (20 μL)
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