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  5. Thermo Scientific™ Oxoid™ Luria Broth (Miller's Modified)

Thermo Scientific™ Oxoid™ Luria Broth (Miller's Modified)

Product Code. 13274843
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Product Code. 13274843 Supplier Thermo Scientific™ Supplier No. CM0996B

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Description

Miller's Modified Luria Broth is used as a microbial growth medium for the cultivation of E. coli strains for the preparation of plasmid DNA and recombinant proteins.

Luria Broth (LB) is a popular, rich medium used to cultivate bacteria and to perform coliphage plaque assays. The original formulation was published in 1951 by Guiseppe Bertani to study phage plaque formation and named Lysogeny Broth. It is also known as Lennox Broth and Luria-Bertani Broth.

Widely used LB medium formulations differ in salt concentration:

  • Miller: 10 g/L NaCl
  • Lennox: 5 g/L NaCl
  • Luria: 0.5 g/L NaCl

Luria Broth Base (Miller's formulation) is recommended for the general maintenance and growth of E. coli as it allows for fast cell expansion and supports high cell density. In cases where salt-sensitive antibiotics are being used for selection (such as Zeocin, tetracycline or chloramphenicol), Lennox formulation medium would be recommended.

Luria Broth Base formulation

Formulation grams per liter: Pancreatic digest of casein 10.0, Yeast extract, 5.0 Sodium chloride 10.0

To prepare:  Suspend 25.0 g in 1 L of distilled water. Mix well and distribute into final containers. Sterilize by autoclaving at 121°C for 15 minutes.
 

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Room temperature
Product Type Microbiology Media
Target Organism Class Coliforms
For Use With (Application) For the cultivation of E. coli strains for the preparation of plasmid DNA and recombinant proteins
pH 7.0 ± 0.2 at 25°C
Quantity 500 g
Species Escherichia coli
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