missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Enzymes and Inhibitors
  4. Restriction Enzymes
  5. OPTIZYME™ SmaI, Fisher BioReagents™

OPTIZYME™ SmaI, Fisher BioReagents™

Product Code. 11934852
Change view
Click to view available options
Quantity:
1200 U
5000 U
Unit Size:
Each
Product Code. Quantity unitSize
11934852 5000 U Each
11944852 1200 U Each
2 options
This item is not returnable. View return policy

Product Code. 11934852

Brand: Fisher BioReagents BP80115

Request Bulk or Custom Format

Please to purchase this item. Need a web account? Register with us today!

This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
 View alternative products

This item is not returnable. View return policy

Description

5'...C C C^G G G...3'
3'...G G G^C C C...5'

Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
  • Incubate at 30°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 50 - 100%
  • Buffer 2: 0 - 20%
  • Buffer 3: 0 - 20%
  • Buffer 4: 100%
  • Buffer 5: 0 - 20%
Storage Buffer:
  • 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 90% of the pUC19 DNA fragments can be ligated and re-cut after 50-fold over-digestion with SmaI.
  • Digestion of Agarose-embedded DNA:
  • A minimum of 5 units of SmaI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
  • Notes:
    • Incubation at 37°C results in 50% activity. SmaI has a half-life of 15 min at 37°C.
    • Incubation at 25°C results in 100% activity.
    • SmaI needs K+ to work for activity.
    Methylation Effects:
    Dam: Never overlaps - no effect
    Dcm: Never overlaps - no effect
    CpG: Completely overlaps - blocked
    • CCCGGG
  • (Cleavage blocked)
  • EcoKI: Never overlaps - no effect
  • EcoBI: Never overlaps - no effect

    • TRUSTED_SUSTAINABILITY

    Specifications

    Concentration 10 U/μL
    Components 10X OPTIZYME™ Buffer 4
    Incubator Temperature 30°C
    pH 7.4
    For Use With (Application) >90% of pUC19 DNA fragments can be ligated and re-cut after 50-fold over-digestion with SmaI
    Storage Buffer 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 50% Glycerol
    Quantity 5000 U
    Cut Site CCC.GGG
    Product Type SmaI
    Form Liquid
    Product Title
    Select an issue

    By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.