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OPTIZYME™ NotI, Fisher BioReagents™
3595.00 NOK - 3940.00 NOK
Specifications
| Concentration | 10 U/μL |
|---|---|
| Components | 25 to 50% Water, >50% Glycerin |
| pH | 7.4 |
| For Use With (Application) | >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Not I |
| Storage Buffer | 20mM Tris HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol |
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|
11944842
|
Fisher Bioreagents
BP8004-5 |
1000 U |
3940.00 NOK
Each |
Estimated Shipment: 23-05-2024 Log in to see stock. |
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|
11934842
|
Fisher Bioreagents
BP8004-1 |
300 U |
3595.00 NOK
Each |
Estimated Shipment: 06-05-2024 Log in to see stock. |
Please sign in to purchase this item. Need a web account? Register with us today! | ||||
Description
5'...G C^G G C C G C...3'
3'...C G C C G G^C G...5'
Conditions for 100% Activity
- 1X OPTIZYME Buffer 3: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2 , 100mM NaCl and 0.1mg/ml BSA.
- Incubate at 37°C.
Enzyme Activity in OPTIZYME buffers
- Buffer 1: 0 - 20%
- Buffer 2: 20 - 50%
- Buffer 3: 100%
- Buffer 4: 0 - 20%
- Buffer 5: 20 - 50%
20mM Tris-HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton X-100, 0.2mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage:
More than 95% of DNA fragments can be ligated and recut after a 50-fold overdigestion with NotI.
Digestion of Agarose-embedded DNA:
A minimum of 5 units of NotI is required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
Compatible Ends: Bsp120I, CfrI, Eco52I.
Methylation Effects:
Dam: never overlaps - no effect.
Dcm: never overlaps - no effect.
CpG: completely overlaps - blocked.
- GCGGCCGC
Specifications
| 10 U/μL | |
| 7.4 | |
| 20mM Tris HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol | |
| GC.GGCCGC | |
| Liquid |
| 25 to 50% Water, >50% Glycerin | |
| >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Not I | |
| Keep container tightly closed | |
| NotI |