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  5. OPTIZYME™ DdeI (HpyF31), Fisher BioReagents™

OPTIZYME™ DdeI (HpyF31), Fisher BioReagents™

Product Code. 11984812
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Quantity:
500 U
Product Type:
DdeI
Unit Size:
Each
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11984812 500 U DdeI Each
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Product Code. 11984812 Supplier Fisher BioReagents Supplier No. BP80601
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Description

5'...C^T N A G...3'
3'...G A N T^C...5'

Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
  • Incubate at 37°C
  • Enzyme Activity in OPTIZYME buffers:
    • Buffer 1: 20 - 50%
    • Buffer 2: 20 - 50%
    • Buffer 3: 20 - 50%
    • Buffer 4: 100%
    • Buffer 5: 20 - 50%

    Storage Buffer:
    • 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycero
    l
  • Ligation and Re-cleavage:
  • More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digesiton with DdeI.
  • Compatible Ends: BbvCI, Bpu10I, Bpu1102I, Eco81I
  • Methylation Effects:
  • Dam: Never overlaps - no effect
  • Dcm: Never overlaps - no effect
  • CpG: Never overlaps - no effect
  • EcoKI: Never overlaps - no effect
  • EcoBI: May overlap - effect not determined

TRUSTED_SUSTAINABILITY

Specifications

Concentration 10 U/μL
Components 25 to 50% Water, >50% Glycerin
Incubator Temperature 37°C
pH 7.4
For Use With (Application) >95% of DNA fragments can be ligated and re-cut after 50-fold over-digesiton with DdeI
Storage Buffer 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Content And Storage Keep container tightly closed
Quantity 500 U
Cut Site C.TNAG
Product Type DdeI
Form Liquid
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