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  5. Macherey-Nagel™ NucleoSpin™ RNA Plant, Mini kit for RNA from plant

Macherey-Nagel™ NucleoSpin™ RNA Plant, Mini kit for RNA from plant

Product Code. 12711021
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No. of Reactions:
10 Preps
250 Preps
50 Preps
Unit Size:
10 reactions
250 reactions
50 reactions
Product Code. No. of Reactions unitSize
12711021 250 Preps 250 reactions
12979894 10 Preps 10 reactions
12721021 50 Preps 50 reactions
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Product Code. 12711021

Brand: Macherey-Nagel™ 740949.250

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This item is not returnable. View return policy

Description

Isolates total RNA from all kinds of plant material and filamentous fungi without degradation of the RNA. Macherey-Nagel™ NucleoSpin™ RNA Plant Kit is a complete mini spin kit that includes RNA plant columns with collection tubes, filters, all needed buffers and RNase-free rDNase.

NucleoSpin™ RNA Plant Kit is designed for isolation of DNA-free, high-quality RNA from a wide variety of plant and fungal samples. Typical downstream applications include real-time RT-PCR, gene expression profiling, Northern blotting, primer extension, array technology, and RNase protection assays.

  • Two different lysis buffers included—Optimized lysis procedure for typical conditions and for peculiar metabolites
  • rDNase included for on-column digestion—Efficient removal of contaminating DNA
  • NucleoSpin™ Filters (shredders) included—Efficient sample homogenization and reduction of viscosity
  • Parallel purification of DNA possible—By using the NucleoSpin™ RNA/DNA buffer set

Kit Components: NucleoSpin™ RNA columns with 2 mL collection tubes, 2 mL and 1.5 mL collecting tubes, NucleoSpin™ filters, buffers (lysis, wash, desalting), RNase-free DNase I, reaction buffer for DNase, RNase-free water

  • Mini spin column with silica membrane technology
  • Yields 3 to 70μg RNA from 100μg plant material
  • Elution volume: 40 to 60μL
  • Fragment size: 200b–20kb
  • Binding capacity: 200μg
  • Preparation time: 30 min./6 preps
  • A260/A280: 1.9–2.1

Specifications

Format Mini Spin Column
For Use With (Equipment) Real-time RT-PCR
For Use With (Application) RNA purification from plant cells and tissue
Includes Lysis buffer RA1 (125mL), Lysis buffer RAP (125mL), Wash buffer RAW2 (80mL), Wash Buffer RA3 concentrate (3 x 25mL), Membrane desalting buffer (125mL), Reaction buffer for rDNase (30mL), RNase-free rDNase (5 size F vials), RNase-free water (60mL), NucleoSpin filters (250), NucleoSpin RNA Plan Columns with collection tubes (250), 2mL Collection tubes (750), 1.5mL Collection tubes (250)
No. of Reactions 250 Preps
Product Type NucleoSpin™ RNA Plant Column
Quantity 1 Each
Sample Size <100 mg
Yield 3–70μg

Safety and Handling

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Signal Word
  • Danger
 Hazard Category
  • Flammable liquid Category 3
  • Acute toxicity Category 4
  • Skin corrosion/irritation Category 2
  • Serious eye damage/eye irritation Category 2
  • Respiratory sensitiser Category 1
  • LONG-TERM AQUATIC HAZARD Chronic 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H412-Harmful to aquatic life with long lasting effects.
 Precautionary Statement
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
 Supplemental information
  • MIXTURE LIST-Contains : ethanol (diluted < 20 %)(denatured with1%IPA/1%MEK, acc.2016/1867/EU); guanidinium thiocyanate; gua
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