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Macherey-Nagel™ NucleoSpin Plasmid QuickPure™ Kit
Description
With the NucleoSpin™ Plasmid QuickPure method, the bacteria culture is lysed according to alkaline lysis. The lysate is cleared by centrifugation and the supernatant is loaded onto the NucleoSpin™ Plasmid QuickPure column. Contaminations like salts, metabolites, nucleases and soluble macromolecular cellular components are removed by only one single washing step with buffer AQ. Pure plasmid DNA is finally eluted under low ionic strength conditions with slightly alkaline buffer AE (5mM Tris-Cl, pH 8.5). The prepared plasmid DNA is suitable for all common downstream applications.
Kit components:
Resuspension Buffer, Lysis Buffer, Neutralization Buffer, Wash Buffer, Elution Buffer, RNase A, NucleoSpin™ Plasmid QuickPure Columns, Collection Tubes, User manual
Features:
- Yield: up to 15μg
- Ultra-fast procedure: 18 minipreps in 11min
- Elution volume: 50μL
- Sample material: 1mL to 3mL E. coli culture
- Very good price-performance ratio
- Silica-membrane technology
- Binding capacity: 15μg
- No use of organic solvents
- Ultra-fast small scale preparation of plasmid DNA
- Highly pure nucleic acids suitable for all common downstream applications
- Vectors <15kb
- Format: mini-spin columns
Specifications
Specifications
| Content And Storage | Room temperature (18–25°C) |
| Format | Mini Spin Column |
| For Use With (Equipment) | Standard benchtop microcentrifuge |
| For Use With (Application) | Plasmid DNA purification/isolation |
| Includes | Resuspension Buffer A1, Lysis Buffer A2 (without LyseControl), Neutralization Buffer A3, Wash Buffer AQ (Concentrate), Elution Buffer AE, RNase A (lyophilized), NucleoSpin™ Plasmid QuickPure Columns (dark yellow rings), Collection Tubes (2mL), User manual |
| No. of Reactions | 250 Preps |
| Product Type | NucleoSpin Plasmid QuickPure |
| RNase | Lyophilized |
| Sample Volume | Culture volume: 1–3mL high copy |
| Size | <15 kbp |
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Safety and Handling
- Danger
- Acute toxicity Category 4
- Skin corrosion/irritation Category 2
- Serious eye damage/eye irritation Category 2
- Respiratory sensitiser Category 1
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
- MIXTURE LIST-Contains : sodium hydroxide solution (diluted < 2 %), dodecyl sulfate,sodium salt
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