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  5. Macherey-Nagel™ NucleoSpin™ miRNA, Mini kit for miRNA and RNA purification

Macherey-Nagel™ NucleoSpin™ miRNA, Mini kit for miRNA and RNA purification

Product Code. 11932512
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No. of Reactions:
10 Reactions
250 Reactions
50 Reactions
Unit Size:
10 reactions
250 reactions
50 reactions
Product Code. No. of Reactions unitSize
11932512 10 Reactions 10 reactions
12788622 250 Reactions 250 reactions
12798622 50 Reactions 50 reactions
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Product Code. 11932512

Brand: Macherey-Nagel™ 740971.10

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This item is not returnable. View return policy

Description

Isolate small and large RNA in parallel. NucleoSpin™ miRNA Kits work on a wide varitey of tissue and cell culture formats.

Features

  • Parallel isolation of small and large RNA
  • RNA purification fractionated by size:
  • Isolation of small RNA only (< 200 b),
  • Isolation of small RNA (< 200 b) and large RNA (> 200 b) in two separate fractions
  • Isolation of total RNA (small and large RNA in one fraction)
  • Additional isolation of total protein fraction ready to use for SDS-PAGE and Western blot analysis
  • Excellent RNA recovery and purity by chaotropic salt lysis without phenol/chloroform (patent pending)
  • NucleoSpin™ Filters for efficient sample homogenization
  • rDNase for efficient on-column removal of genomic DNA

Applications

  • Parallel isolation of small and large RNA from human / animal tissue and cultured cells, from plant tissue, and in combination with phenol / chloroform (e.g., TRIzol™) lysis
  • Purification of siRNA and large dsRNA from DICER reactions
  • Typical downstream applications: real-time RT-PCR, Northern blotting, chip hybridization

Specifications

Format Mini Spin Column
For Use With (Application) Purification of siRNA and large dsRNA from DICER reactions
Includes NucleoSpin™ RNA Columns, NucleoSpin™ miRNA Columns, NucleoSpin™ Protein Removal Columns, Collection Tubes (2 mL, 2 mL lid, 1.5 mL), NucleoSpin™ Filters, buffers, RNase-free rDNase
No. of Reactions 10 Reactions
Product Type NucleoSpin™ miRNA Kit
RNase Yes
Sample Size Small RNA: < 200 nt, large RNA: > 200 nt
Test Time <45 min./6 Reaction and <35 min./6 Reaction
Yield 10μg small RNA, 95μg large RNA

Safety and Handling

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Signal Word
  • Danger
 Hazard Category
  • Acute toxicity Category 4
  • LONG-TERM AQUATIC HAZARD Chronic 2
  • LONG-TERM AQUATIC HAZARD Chronic 3
  • Carcinogenicity Category 2
  • Serious eye damage/eye irritation Category 1
  • Serious eye damage/eye irritation Category 2
  • Flammable liquid Category 2
  • Flammable liquid Category 3
  • Respiratory sensitiser Category 1
  • Skin corrosion/irritation Category 1 B
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H225-Highly flammable liquid and vapour.
  • H226-Flammable liquid and vapour.
  • H302-Harmful if swallowed.
  • H314-Causes severe skin burns and eye damage.
  • H318-Causes serious eye damage.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H335-May cause respiratory irritation.
  • H351-Suspected of causing cancer.
  • H411-Toxic to aquatic life with long lasting effects.
  • H412-Harmful to aquatic life with long lasting effects.
 Precautionary Statement
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P284-¢In case of inadequate ventilation! wear respiratory protection.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P310-Immediately call a POISON CENTER/doctor/.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : 1,4-dioxane, ethanol, guanidinium thiocyanate, 2-mercaptoethanol, rDNase
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