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  5. Macherey-Nagel™ NucleoSpin™ Microbial DNA Mini kit for DNA from microorganisms

Macherey-Nagel™ NucleoSpin™ Microbial DNA Mini kit for DNA from microorganisms

Product Code. 15353609
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No. of Reactions:
10
50
250
Unit Size:
10 reactions
250 reactions
50 reactions
This item is not returnable. View return policy

Product Code. 15353609

Brand: Macherey-Nagel™ 740235.10

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This item is not returnable. View return policy

Description

Kit for reliable isolation of DNA from microorganisms (e.g. yeast, fungi, and gram-negative and gram-positive bacteria), with lysis that is supported by mechanical disruption with glass beads (tubes included).

The NucleoSpin™ Microbial DNA kit is designed for convenient, fast, and easy isolation of genomic DNA from microbial samples. Because microbial samples can be difficult to lyse (due to their strong complex cell wall structures), the kit replaces enzymatic lysis with mechanical disruption of cell wall structures utilizing NucleoSpin™ Bead Tubes.

  • Suitable for a large variety of starting materials
  • High yields of genomic DNA can be isolated
  • NucleoSpin Bead Tubes Type B included – for efficient lysis, compatible with many disruption devices
  • Fast and easy procedure – Liquid Proteinase K included, no additional enzyme required
  • Typical downstream applications: PCR, real-time PCR, southern blotting, enzymatic reactions

Specifications

Format Mini Spin Column
Isolation Technology Silica MembraneTechnology and Mechanical (Bead Tube) Disruption
Sample Type Bacteria (Gram +/-), Fungi, Yeast
Efficiency Binding capacity: 60 μg
Elution Volume Elution volume: 100 - 200 μL
Final Product Type Genomic DNA
For Use With (Equipment) Centrifuge, Vortex mixer
For Use With (Application) DNA isolation from microorganisms
Includes NucleoSpin Microbial DNA Columns, NucleoSpin Bead Tubes Type B, Collection Tubes (2 mL), buffers, Liquid Proteinase K
No. of Reactions 10
Purity A260/A280: 1.6 - 2.0
Sample Volume <40 mg wet weight
Test Time 35 min/prep
Yield 5 - 25 μg
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Safety and Handling

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Product Identifier
  • NucleoSpin Microbial DNA
 Signal Word
  • Warning
 Hazard Category
  • Acute toxicity Category 4
  • LONG-TERM AQUATIC HAZARD Chronic 3
  • Serious eye damage/eye irritation Category 2
  • Flammable liquid Category 3
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • H302-Harmful if swallowed.
  • H319-Causes serious eye irritation.
  • H336-May cause drowsiness or dizziness.
  • H412-Harmful to aquatic life with long lasting effects.
 Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash with water thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P330-Rinse mouth.
 Supplemental information
  • MIXTURE LIST-Contains : guanidine hydrochloride; 2-propanol; proteinase K, liquid (origin: tritirachiumalbum); guanidinium
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