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Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up, kit for gel extraction or PCR clean up

Product Code. p-8013747
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No. of Reactions:
240 Preps
50 Preps
Unit Size:
240 preps
50 reactions
This item is not returnable. View return policy

Product Code. 15116456

Brand: Macherey-Nagel™ 740609.240C

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This item is not returnable. View return policy

Clean up PCR reactions and gel extractions with a two-in-one approach. Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit is optimized for recovery and elution volume when purifying DNA fragments via both applications.

NucleoSpin™ Gel and PCR Clean-up is designed for fast purification of PCR products, such as DNA from enzymatic reactions, as well as the extraction of DNA fragments from TAE or TBE agarose gels.

  • Two applications in one kit—One buffer to process both sample types, with only one loading step
  • High recovery for fragments down to 50 bp—Average recovery from 60–90%
  • Minimized elution volume of 15μL—Highly concentrated DNA
  • Binding buffer with pH indicator—Ensures optimal binding conditions
  • Separate buffers for single stranded DNA and RNA or for SDS-containing samples available separately
  • Suitable for all kinds of agarose gels and a variety of buffer systems (e.g. TAE, TBE)
  • Complete removal of contaminations: nucleotides, primers, enzymes, mineral oil, PCR additives, detergents, dyes
  • Applications: purification of PCR products, extraction of DNA from agarose or polyacrylamide gels, and downstream applications (cloning, sequencing, PCR, restriction analysis)

Kit contents: NucleoSpin™ Gel and PCR Clean-up columns, collection tubes (2mL), binding buffer with pH indicator, wash buffer and elution buffer

Spécification

Color pH Indicator:
Yellow = correct conditions
Green = pH slightly high (adjustment recommended)
Blue = pH too high (adjustment necessary)
Format Mini Spin Column
Isolation Technology Silica Membrane Technology
Efficiency Typical recovery: 70–95%
Elution Volume Elution volume: 30–100μL
For Use With (Equipment) QIAcube™ compatible
TAE or TBE agarose gels, PCR
Includes Binding Buffer NTI (2 x 75mL), Wash Buffer NT3 Concentrate (60mL), Elution Buffer NE (125mL), NucleoSpin Gel and PCR Clean-up Columns (4 x 60)
No. of Reactions 240 Preps
Quantity 1 Kit
Purity A260/A280: 1.8–1.9
Sample Volume <400 μL PCR reaction mixture, <400 μg agarose gel
Size 50 bp to ∼20 kbp
Test Time 10 min/6 preps
Yield Up to ∼15μg DNA
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Mention d'avertissement
  • Warning
Catégorie de danger
  • Acute toxicity Category 4
  • LONG-TERM AQUATIC HAZARD Chronic 3
Mention de danger
  • H302-Harmful if swallowed.
  • H412-Harmful to aquatic life with long lasting effects.
Informations supplémentaires
  • MIXTURE LIST-Contains : guanidinium thiocyanate
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