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  5. Macherey-Nagel™ NucleoSpin™ gDNA Clean-up, Mini kit for DNA clean up and concentration

Macherey-Nagel™ NucleoSpin™ gDNA Clean-up, Mini kit for DNA clean up and concentration

Product Code. 12783391
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No. of Reactions:
10
50
250
Unit Size:
Each
Pack of 10
Product Code. No. of Reactions unitSize
12783391 50 Each
12773391 250 Each
12763391 10 Pack of 10
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Product Code. 12783391

Brand: Macherey-Nagel™ 740230.50

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This item is not returnable. View return policy

Description

Concentrate DNA samples more easily and quickly, compared to microdialysis filtrations units. Macherey-Nagel™ NucleoSpin™ gDNA Clean-up Kit cleans and concentrates large-sized genomic DNA from pre-purified DNA from organic extraction and from enzymatic extractions.

The NucleoSpin™ gDNA Clean-up kit is designed for the rapid purification of previously isolated small and especially high molecular weight genomic DNA. It is used to clean-up and concentrate genomic DNA after crude extraction methods, for example using Trizol, or after enzymatic, or chemical reactions. The time-saving procedures enables a preparation time of just 15 minutes for 10 preps.

  • No need for organic denaturants or chloroform extractions
  • Any impurities like phenol, enzymes, salts, dyes, labels, nucleotides, small oligonucleotides, and even up to 5% detergents (e.g., SDS, Triton, Tween, Lauroylsarcosin) are removed completely
  • Binding Buffer DB and Wash Buffer DW are developed to allow a very gentle binding and washing
  • High recovery rates up to 90% for high molecular weight DNA (up to 50 kbp) as well as for small DNA fragments down to 100 bp
  • The eluted DNA is ready to use for downstream applications such as PCR, endonuclease restriction, Southern Blotting and labeling
Kit components:
  • NucleoSpin™ gDNA Clean-up Columns, Collection Tubes (2 mL), Binding Buffer DB, Wash Buffer DW, Elution Buffer DE

Specifications

Format Mini Spin Column
Isolation Technology Silica Membrane Technology
Sample Type DNA (Genomic)
Efficiency Recovery rate: 80–90%
Elution Volume Elution volume: 50–100μL
Final Product Type Nucleo Spin gDNA Clean Up
Includes NucleoSpin™ gDNA Clean-up Columns (50), 2mL Collection Tubes (50), Binding Buffer DB (25mL), Wash Buffer DW Concentrate (25mL), Elution Buffer DE (13mL)
No. of Reactions 50
Product Type Nucleo Spin gDNA Clean Up
Purity A260/A280: 1.8–1.9
Sample Volume <150 μL solution containing <25 μg DNA
Test Time <15 min/10 preps
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Safety and Handling

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Signal Word
  • Danger
 Hazard Category
  • Flammable liquid Category 2
 Hazard Statement
  • H225-Highly flammable liquid and vapour.
 Supplemental information
  • MIXTURE LIST-Contains : ethanol
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