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  5. Macherey-Nagel™ NucleoSpin™ DNA Stool, Mini kit for DNA from stool

Macherey-Nagel™ NucleoSpin™ DNA Stool, Mini kit for DNA from stool

Product Code. 15859097
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No. of Reactions:
10
50
250
Unit Size:
10 reactions
250 reactions
50 reactions
This item is not returnable. View return policy

Product Code. 15859097

Brand: Macherey-Nagel™ 740472.250

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This item is not returnable. View return policy

Description

Combines enzymatic lysis with mechanical disruption of cell walls for efficient isolation of high-quality genomic DNA from any stool sample, fresh or frozen. Complete kit includes NucleoSpin™ Bead Tubes.

Isolation of genomic DNA from bacterial and epithelial cells from stool samples

  • Efficient procedure combining enzymatic lysis and bead disruption
  • Isolation of high-quality genomic DNA (microbial and host) from fresh and frozen samples
  • Suitable for any stool sample – carnivores, omnivores, and herbivore
  • Bead Tubes included for efficient lysis – compatible with most common disruption devices
  • NucleoSpin™ Inhibitor Removal Columns ensure highest purity of the isolated DNA
  • Downstream applications: PCR, real-time PCR, Southern blotting, microarray technology

Specifications

Content And Storage Room temperature (18 - 25 °C)
Format Mini Spin Column
Isolation Technology Silica MembraneTechnology and Mechanical (Bead Tube) Disruption
Sample Type Stool Samples (Herbivores, Omnivores, or Carnivores, Fresh or Frozen)
Efficiency Binding capacity: 50 μg
Elution Volume Elution volume: 30 - 100 μL
For Use With (Equipment) Disruption device (e.g. Vortex-Genie 2 with MN Bead Tube Holder), Centrifuge, Vortex mixer
For Use With (Application) Isolation of genomic DNA from stool samples
Includes DNA Stool Columns, Bead Tubes Type A, Inhibitor Removal Columns, Collection Tubes (2 mL), Collection Tubes (2 mL, lid), Buffers
No. of Reactions 250
Purity A260/A280: 1.7 - 1.9
Sample Volume Approx. 200 mg (180 - 220 mg)
Size Fragment size: 200 bp - approx. 50 kbp
Test Time 60 min/10 prep
Yield Varies by sample type, quality and water content
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Safety and Handling

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Product Identifier
  • NucleoSpin DNA Stool
 Signal Word
  • Warning
 Hazard Category
  • Acute toxicity Category 4
  • LONG-TERM AQUATIC HAZARD Chronic 3
  • Serious eye damage/eye irritation Category 2
  • Flammable liquid Category 3
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H336-May cause drowsiness or dizziness.
  • H412-Harmful to aquatic life with long lasting effects.
 Supplemental information
  • MIXTURE LIST-Contains : 2-propanol, guanidine hydrochloride
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