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  5. Macherey-Nagel™ NucleoBond™ PC EF, kit for endotoxin-free plasmid DNA

Macherey-Nagel™ NucleoBond™ PC EF, kit for endotoxin-free plasmid DNA

Product Code. 12873971
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No. of Reactions:
5
10
Yield:
100–500μg
2,000–10,000μg
500–2,000μg
Sample Volume:
30 - 150mL
150 - 500mL
500 - 2000mL
Test Time:
100 min./2 preps
150 min./2 preps
180 min./2 preps
Unit Size:
10 reactions
5 preps
5 reactions
Product Code. No. of Reactions Yield Sample Volume Test Time unitSize
12873971 5 2,000–10,000μg 500 - 2000mL 180 min./2 preps 5 preps
12863931 10 100–500μg 30 - 150mL 100 min./2 preps 10 reactions
12860661 5 500–2,000μg 150 - 500mL 150 min./2 preps 5 reactions
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Product Code. 12873971

Brand: Macherey-Nagel™ 740548

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This item is not returnable. View return policy

Description

Removes endotoxins via an additional washing step, without ice incubation. Macherey-Nagel™ NucleoBond™ PC EF Kit is a classic anion exchanger for purification of endotoxin-free plasmid DNA. The kit includes NucleoBond™ AX Columns, all buffers, RNase A and NucleoBond™ Filters.

An important prerequisite for transfections and gene therapy applications is endotoxin-free plasmid DNA. The patented procedure of NucleoBond™ PC EF reduces endotoxins to a very low level using the same working procedure as the standard protocol. In comparison to competitive kits, there is no need for a time-consuming incubation step to remove the endotoxins.

Features

  • Fast and easy clarification by NucleoBond™ Folded Filters—No centrifugation required, no shearing forces
  • Proven anion-exchange technology—Yields transfection-grade high-copy plasmid DNA from E. coli
  • NucleoBond™ Columns contain NucleoBond™ AX Silica Resin packed between inert filters
  • Polypropylene columns are resistant to organic solvents and are free of DNase and RNase
  • AX Resin can be used over a wide pH range and can remain in contact with buffers for up to three hours

Specifications

  • DNA with less than 0.1 EU/μg DNA
  • Purity of plasmid DNA: A260/A280 = 1.80–1.95
  • Recovery of plasmid DNA >90%
  • Structural integrity >90%
  • Yield between 500μg up to 10mg, depending on kit used

Specifications

Concentration Endotoxin level: <0.1 EU/μg
Format Gravity-flow Column (Giga)
Isolation Technology Anion Exchange Chromatography Technology
Efficiency Recovery of plasmid DNA: >90%
For Use With (Application) High-copy plasmids from E. coli
Includes NucleoBond AX 10000 Columns, buffers, RNase A, NucleoBond Bottle Top Filters
No. of Reactions 5
Product Type NucleoBond™ PC 10,000 EF
Quantity 1 Kit
Purity A260/A280: 1.80–1.95
RNase Yes
Sample Volume 500 - 2000mL
Size <300 kbp
Test Time 180 min./2 preps
Yield 2,000–10,000μg
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Safety and Handling

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Signal Word
  • Danger
 Hazard Category
  • Flammable liquid Category 3
  • Skin corrosion/irritation Category 2
  • Serious eye damage/eye irritation Category 2
  • Respiratory sensitiser Category 1
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
 Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
 Supplemental information
  • MIXTURE LIST-Contains : ethanol (diluted < 20 %)/sodium hydroxide solution (diluted< 2 %)/dodecyl sulfate, sodium salt
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