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Macherey-Nagel™ NucleoBond™ PC EF, kit for endotoxin-free plasmid DNA
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No. of Reactions:
5
10
Yield:
100–500μg
2,000–10,000μg
500–2,000μg
Sample Volume:
30 - 150mL
150 - 500mL
500 - 2000mL
Test Time:
100 min./2 preps
150 min./2 preps
180 min./2 preps
Unit Size:
10 reactions
5 preps
5 reactions
Description
An important prerequisite for transfections and gene therapy applications is endotoxin-free plasmid DNA. The patented procedure of NucleoBond™ PC EF reduces endotoxins to a very low level using the same working procedure as the standard protocol. In comparison to competitive kits, there is no need for a time-consuming incubation step to remove the endotoxins.
Features
- Fast and easy clarification by NucleoBond™ Folded Filters—No centrifugation required, no shearing forces
- Proven anion-exchange technology—Yields transfection-grade high-copy plasmid DNA from E. coli
- NucleoBond™ Columns contain NucleoBond™ AX Silica Resin packed between inert filters
- Polypropylene columns are resistant to organic solvents and are free of DNase and RNase
- AX Resin can be used over a wide pH range and can remain in contact with buffers for up to three hours
Specifications
- DNA with less than 0.1 EU/μg DNA
- Purity of plasmid DNA: A260/A280 = 1.80–1.95
- Recovery of plasmid DNA >90%
- Structural integrity >90%
- Yield between 500μg up to 10mg, depending on kit used
Specifications
Specifications
| Concentration | Endotoxin level: <0.1 EU/μg |
| Format | Gravity-flow Column (Giga) |
| Isolation Technology | Anion Exchange Chromatography Technology |
| Efficiency | Recovery of plasmid DNA: >90% |
| For Use With (Application) | High-copy plasmids from E. coli |
| Includes | NucleoBond AX 10000 Columns, buffers, RNase A, NucleoBond Bottle Top Filters |
| No. of Reactions | 5 |
| Product Type | NucleoBond™ PC 10,000 EF |
| Quantity | 1 Kit |
| Purity | A260/A280: 1.80–1.95 |
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Safety and Handling
Signal Word
- Danger
- Flammable liquid Category 3
- Skin corrosion/irritation Category 2
- Serious eye damage/eye irritation Category 2
- Respiratory sensitiser Category 1
- H226-Flammable liquid and vapour.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- MIXTURE LIST-Contains : ethanol (diluted < 20 %)/sodium hydroxide solution (diluted< 2 %)/dodecyl sulfate, sodium salt
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