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Macherey-Nagel™ NucleoBond™ PC EF Kit Buffer Solutions
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Quantity:
600 mL
1000 mL
Chemical Name or Material:
Acetate buffer solution
Elution Buffer N5-EF
Equilibration Buffer N2-EF
Lysis Buffer S2-EF
Resuspension Buffer S1-EF
Wash Buffer N3-EF
Wash Buffer N4-EF
Water
Unit Size:
1 L
600mL
Description
NucleBond™ PC EF: The classic anion exchanger for purification of endotoxin-free plasmid DNA
- Proven anion-exchange technology – transfection-grade plasmid DNA
- Plasmid DNA with less than 0.1 EU/μg for transfection of highly sensitive cells
- Patented endotoxin removal by additional washing step – no extra ice incubation necessary
- Fast and easy clarification of lysates by Folded Filters – no centrifugation required, no shearing forces
- NucleoBond™ Finalizers available to avoid time consuming centrifugation for plasmid precipitation
- Separate binding columns of NucleoBond™ PC kits available as NucleoBond™ AX columns
Specifications
Specifications
| Chemical Name or Material | Wash Buffer N3-EF |
| CAS | 64-17-5d |
| Color | Colorless |
| Concentration | Ethanol, diluted < 20%; Potassium chloride; Water |
| For Use With (Application) | Purification of endotoxin-free plasmid DNA |
| For Use With (Equipment) | NucleoBond™ PC EF kits (PC 500 EF, PC 2000 EF, PC 10000 EF, PC Prep 100) |
| pH | 6.0 to 6.5 |
| Physical Form | Liquid |
| Quantity | 1000 mL |
| Formulation | Ethanol, diluted < 20%; Potassium chloride; Water |
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Safety and Handling
Product Identifier
- Buffer N3-EF (1000 mL)
- Warning
- LONG-TERM AQUATIC HAZARD Chronic 3
- Flammable liquid Category 3
- H226-Flammable liquid and vapour.
- H411-Toxic to aquatic life with long lasting effects.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P273-Avoid release to the environment.
- MIXTURE LIST-Contains : ethanol, triton-x 114
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