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Macherey-Nagel™ NucleoBond™ PC 10000, Giga kit for transfection-grade plasmid DNA

Product Code. 12877521
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Quantity:
1 Kit
Unit Size:
5 reactions
This item is not returnable. View return policy

Product Code. 12877521

Brand: Macherey-Nagel™ 740593

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This item is not returnable. View return policy

Description

1st generation anion exchanger for purification of plasmid DNA.

NucleoBond™ kits utilize anion-exchange chromatography with optimized lysis buffer volumes for plasmid DNA purification. PC 10,000 kits are recommended for the isolation of high-copy plasmids (>20 copies/cell). Low-copy plasmids (<20 copies/cell) can be isolated as well, but this requires additional buffers.

  • Folded Filters are included for fast and easy clarification of lysates with no centrifugation required and no shearing forces
  • Contains sufficient buffer to perform 5 giga preps, with typical yields of 2,000–10,000μg of plasmid DNA from E. coli culture
  • Columns are resistant to organic solvents and are free of DNase and RNase
  • AX Resin can be used over a wide pH range (2.5–8.5) and can remain in contact with buffers for up to three hours

Specifications

Buffer Yes
Format Gravity-flow Column (Giga)
Isolation Technology Anion Exchange Chromatography Technology
Sample Type E. coli Culture
For Use With (Application) Purification of high-copy and low-copy plasmids
Includes Resuspension Buffer S1 (750mL), Lysis Buffer S2 (750mL), Neutralization Buffer S3 (750mL), Equilibration Buffer N2 (500/125mL), Wash Buffer N3 (1000/125mL), Elution Buffer N5 (500/125mL), RNase A lyophilized (75mg), NucleoBond AX 10000 Columns (5), NucleoBond Folded Filters Type 1 (10), NucleoBond Folded Filters Type 2 (10)
No. of Reactions 5 Preps
Product Type PC 10000 Columns
Quantity 1 Kit
Purity A260/A280: 1.80–1.95
Sample Volume 500—2000mL (HC)
Size <300 kbp
Test Time 120–150 min/2 preps
Yield 2,000–10,000μg
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Safety and Handling

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Hazard Category
  • Flammable liquid Category 3
  • Substances and mixtures corrosive to metals Category 1
  • Skin corrosion/irritation Category 2
  • Respiratory sensitiser Category 1
  • Serious eye damage/eye irritation Category 2
  • Skin sensitiser Category 1
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