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MP Biomedicals™ Nuclease P1, MP Biomedicals™

Product Code. 15732262 Shop All MP Biomedicals Products
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15732262 1 Vial Each
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Product Code. 15732262

Brand: MP Biomedicals™ 0218055501

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Description

Exhibits both phosphodiesterase and monoesterase activities. Nuclease P1, MP Biomedicals is a single strand-specific nuclease that exhibits high selectivity for single-stranded nucleic acids and single-stranded regions in double-stranded nucleic acids. De

Catalyzes the nonspecific endonucleolytic cleavage of single stranded DNA and RNA to yield nucleoside 5′-phosphates and 5′-phosphooligonucleotides. It does not appreciably degrade double-stranded nucleic acids, especially in the presence of more than 400 mM sodium chloride at pH 6.0.

  • Useful at removing single stranded strands hanging off the end of double stranded DNA and at completely cleaving melted DNA for simple DNA composition analysis
  • Improves the sensitivity of a 32P-labeling method for the detection of DNA adducts
  • Used in a study to assess crystal structures using ammonium sulphate or polyethylene glycol 4000 as a precipitating agent
  • Used in a study to investigate a method for the direct sequence analysis 20-25 nucleotides from the terinini of 5′ or 3′ end group labeled RNA
TRUSTED_SUSTAINABILITY

Specifications

Color White
pH Optimum pH: 5.3 (RNA and heat-denatured DNA) and 7.2 (3′-AMP) (Lit.)
Content And Storage 4°C
Quantity 1 Vial
Form Solid
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