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PeproTech NTF3 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™
Description
AA Sequence of recombinant protein: MYAEHKSHRG EYSVCDSESL WVTDKSSAID IRGHQVTVLG EIKTGNSPVK QYFYETRCKE ARPVKNGCRG IDDKHWNSQC KTSQTYVRAL TSENNKLVGW RWIRIDTSCV CALSRKIGRT. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human NT-3. Anti-Human NT-3-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hNT-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human NT-3 (500-P82G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hNT-3. Western Blot: To detect hNT-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hNT-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P82BT-1MG will be provided as 2 x 500 μg
Specifications
Specifications
| Antigen | NTF3 |
| Applications | ELISA, Western Blot |
| Classification | Polyclonal |
| Concentration | 0.1-1.0 mg/mL |
| Conjugate | Biotin |
| Formulation | PBS with no preservative |
| Gene | Ntf3 |
| Gene Accession No. | P20783 |
| Gene Alias | AI316846; AI835689; HDNF; Nerve growth factor 2; neurotrophic factor; neurotrophin 3; neurotrophin-3; neurotrophin-3 (HDNF/NT-3); NGF2; NGF-2; NT3; NT-3; NTF3; Ntf-3 |
| Gene Symbols | Ntf3 |
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