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Invitrogen™ IL1RA Human Matched Antibody Pair

Product Code. 10083293
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10 x 96 Tests
Product Code.
10083293 10 x 96 Tests
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Product Code. 10083293

Brand: Invitrogen™ CHC1183

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This item is not returnable. View return policy

Description

Includes

Antibody Pair Kit contains matched, pre-titred and fully optimized Capture (coating) and Detection Antibodies, 3 vials of Recombinant Standard, Streptavidin-HRP concentrate and technical data sheet.

Requires

Buffer reagents required to complete reaction available separately (Cat. No. CNB0011).

Matched Antibody Pair

The Human IL-1RA; Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-1RA. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Interleukin 1 receptor antagonist (ILRA) is a protein related to the IL-1 family based on its similarities in amino acid sequence to both IL-1 beta and IL-1 alpha, similarities in gene structure, and collective gene localization. The human gene contains four exons and maps to chromosome 2q13-14. IL-1 RA binds to the IL-1 receptor and blocks IL-1 action through competitive inhibition. In contrast to IL-1, the binding of IL-1RA does not initiate signaling events. The secretion of IL-1 RA is mediated by a classical signal sequence while neither IL-1alpha nor IL-1beta possess such a sequence. The lack of homology between IL-1 RA and IL-1 alpha or IL-1 beta near the N terminus may provide an explanation for the biosynthetic and functional differences between IL-1 RA and the two IL-1 proteins. The production of IL-1RA is stimulated by many substances including adherent IgG, cytokines, and bacterial or viral components. IL-1RA is an important natural anti-inflammatory protein that when missing or just available in inadequate amounts plays a key role in several pathological conditions such as rheumatoid arthritis, colitis, osteomyelitis, periostitis and pustulosis.

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TRUSTED_SUSTAINABILITY

Specifications

Gene Accession No. P18510
Gene Alias DIRA, ICIL-1RA, IL-1RN, IL-1ra, IL-1ra3, IL1F3, IL1RA, IRAP, MGC10430, MVCD4
Gene Symbols IL1RN
Quantity 10 x 96 Tests
Gene ID (Entrez) 3557
Includes Capture (coating) Antibody, Detection Antibody, Standard, Streptavidin-HRP
Target Species Human
Content And Storage 2°C to 8°C
Product Type ELISA Kit
For Use With (Equipment) Colorimetric Microplate Reader

For Research Use Only. Not for use in diagnostic procedures.

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