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Thermo Scientific™ Muller-Kauffmann Tetrathionate-Novobiocin (MkTTn) Enrichment Broth Tubes
Selectively enrich Salmonella species from food with the convienience of Muller-Kauffmann Tetrathionate-Novobiocin Enrichment Broth prepared media tubes.
Brand: Thermo Scientific™ TV5065E
1081.61 NOK valid until 2024-12-31
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Additional Details : Weight : 0.01000kg
Description
Selectively isolate Salmonella species with the convenience of Thermo Scientific™ Muller-Kauffmann Tetrathionate-Novobiocin (MkTTn) Enrichment Broth prepared media tubes. MkTTn is a selective enrichment medium for the isolation of Salmonella, whose formulation conforms to ISO 6579:20021 for the microbiological testing food and animal feeding stuffs.
Muller-Kauffmann Tetrathionate-Novobiocin Broth was developed by Muller2 and later modified by Kauffmann3,4 with the addition of ox bile and brilliant green to improve selectivity. The addition of novobiocin at 40 mg per liter was later described by Jeffries5 to improve inhibition of Proteus species.
After the sample has been incubated in Buffered Peptone Water (ISO), transfer 1 ml of the broth to 10 ml of MkTTn Broth and 0.1 ml into 10 ml of RVS Broth (TV5036E). Incubate the MkTTn Broth at 37°C ± 1°C for 24 hours ± 3 hours and the RVS Broth at 41.5°C ± 1°C, for 24 hours ± 3 hours. Sub-culture the incubated MkTTn and RVS broths onto XLD Agar (PO5057A/PO0164A) and a second agar medium of choice, and incubate for a further 24 hours ± 3 hours at 37°C ± 1°C. Presumptive Salmonella should be confirmed using appropriate biochemical and serological techniques. For the complete method, please refer to ISO 6579:20021.
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Specifications
| Light green, opaque | |
| Muller Kauffmann Tetrathionate Novobiocin Enrichment Broth | |
| Germany | |
| 10 mL | |
| 50 tubes in a carton box |
| 2°C to 12°C | |
| Tube | |
| Microbiology Media | |
| 10 ± 1mL | |
| 50/Pk. |
For laboratory use only.