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Invitrogen™ MTCO1 Monoclonal Antibody (1D6E1A8)
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Product Code. 10444035
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Quantity:
100 μg
Unit Size:
Each
Product Code. Quantity unitSize
10444035 100 μg Each
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Product Code. 10444035

Brand: Invitrogen™ 459600

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Description

Mouse Monoclonal Antibody

For best results with this antibody in Western blot, do not boil samples before loading onto the gel. Boiling of the sample will cause a loss of signal. Hydrophobic intrinsic membrane proteins such as the core mtDNA-encoded proteins of the mitochondrial OXPHOS complexes tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio. In Western blot applications, this antibody detects a band at approximately 40 kDa (predicted MW: 57 kDa). In mouse liver lysate a specific band below 37 kDa was detected. Recommended positive controls: ICC/IF - Pig retinal pigment epithelial cells, rat cerebellum primary cells IHC (P) - Human skeletal muscle and colon tissue, rat pancreas tissue, pig smooth muscle tissue WB - Mouse, human, bovine and rat heart mitochondria lysate Flow - HEK-293 cells Recommended long term storage at -20°C.

Cytochrome c oxidase subunit I (CO1 or MTCO1) is 1 of 3 mitochondrial DNA (mtDNA) encoded subunits (MTCO1, MTCO2, MTCO3) of respiratory Complex IV. Complex IV is located within the mitochondrial inner membrane and is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. It is composed of 13 polypeptides. Subunits I, II, and III (MTCO1, MTCO2, MTCO3) are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc, and VIII are nuclear encoded. The cytochrome c oxidase family of enzymes have 4 redox centers, 2 hemes and 2 copper centers. In mitochondrial Complex IV, the 2 hemes are a and a3 and the 2 coppers are CuA and CuB. The 2 hemes and CuB are bound to subunit I. Acin-Perez et al. (2003) identified a cell line containing single and double missense mutations in the cytochrome c oxidase (COX) subunit I gene of mouse mitochondrial DNA. And they hypothesized that deleterious mutations can arise and become predominant; cultured cells can maintain several mtDNA haplotypes at stable frequencies; the respiratory chain has little spare COX capacity; and that the size of a cavity in the vicinity of val421 in MTCO1I of animal COX may affect the function of the enzyme.
TRUSTED_SUSTAINABILITY

Specifications

Antigen MTCO1
Applications Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
Classification Monoclonal
Clone 1D6E1A8
Concentration 0.99 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.05% BSA, 40% glycerol and 0.01% sodium azide
Gene mt-Co1
Gene Accession No. P00395, P00397, P05503
Gene Alias Co1; coi; cox1; coxi; Cytochrome c oxidase polypeptide I; Cytochrome c oxidase subunit 1; cytochrome c oxidase subunit I; mitochondrially encoded cytochrome c oxidase I; mtco1; mt-co1
Gene Symbols mt-Co1
Host Species Mouse
Immunogen Human Mitochondrial Complex IV subunit I.
Purification Method Protein A
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 17708, 26195, 4512
Target Species Human, Mouse, Rat
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Product Type Antibody
Form Liquid
Isotype IgG2a κ
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