Invitrogen™ Image-iT™ Fixation/Permeabilization Kit

• Components help preserve cell morphology and reduce background staining and come in practical box with single-use vials and easy-to-follow protocols
• Complete fixation/permeabilization kit with ready-to-use components and room temperature storage
• Optimized for secondary antibody labeling, fixed-cell dye staining, and GFP applications
• Convenient storage box, 1X ready-to-use components
• Compatible with analysis of most cellular antigens
• Reduced background staining
• Components were chosen to meet wide range of sample preparation requirements demanded for successful fluorescent imaging of fixed and permeabilized cell
• Reagents optimaized for both preservation of 3D cellular structure and adequate antibody access to intracellular antigenic sites
• Kit components were extensively tested with both nuclear and cytoplasmic targets and showed equal or superior results compared to standard reagents
• Cells are treated sequentially with fixative, permeabilization solution, and blocking buffer according to simplified and easy-to-follow protocol: This procedure affords optimal access to intracellular structures for antibodies and fixed cell dyes and maintains cellular morphology for subsequent fluorescent cell imaging
• Decreased background staining, consequently improving signal-to-noise levels and image quality

Cell Analysis, Cellular Imaging, IHC Staining and Detection, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunofluorescence Sample Preparation, Immunohistochemistry (IHC)