Invitrogen™ Fluo-5N, Pentapotassium Salt, cell impermeant

Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca²⁺.

• Cells may be physically loaded with cell-impermeant salt forms of these indicators using patch pipet or microinjection or Influx™ pinocytotic cell-loading reagent
• Compatible with excitation at 488nm by argon-ion laser sources, making them useful for confocal microscopy, flow cytometry, and microplate screening applications
• Exhibit fluorescence increase upon binding Ca²⁺ with little shift in wavelength
• BAPTA-based indicators bind various heavy metal cations (e.g., Mn²⁺, Zn²⁺, Pb²⁺) with substantially higher affinity than Ca²⁺
• Perturbations to calcium measurements caused by presence of the ions can be controlled using heavy metal-selective chelator

• Label (Ex/Em of Ca²⁺-bound form): Fluo-5N (494/516nm)
• Fluorescence intensity increase upon binding Ca²⁺: >100 fold
• K_d for Ca²⁺ in buffer: approximately 90μM

Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling