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IBA Lifesciences pCSG-IBA Mammalian Expression Vector with No Secretion
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Beschreibung
pCSG-IBA mammalian expression vectors
- Designed for high-level episomal expression in mammalian hosts under G418 selection containing the following elements:
- Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
- Episomal replication through Epstein Barr Virus replication origin (oriP) and nuclear antigen encoded by EBNA-1 in human, primate and canine cells and through SV40 replication origin in cells latently infected with SV40 or that express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7)
- Stable cell lines can be selected by the neomycin resistance gene (NeoR).
- In addition, the human cytomegalovirus (CMV) immediate-early promoter enables a high-level expression in a wide range of mammalian cells.
Please note that cloning into pCSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. Besides to the direct cloning of the gene of interest into pCSG-IBA vectors with Esp3I, another option via a so-called entry-vector is possible.
Spezifikation
Spezifikation
| Constitutive or Inducible System | Constitutive |
| Marker Gene | Ampicillin |
| Promoter | CMV |
| Product Type | Vector |
| Selection Agent (Eukaryotic) | Geneticin™ (G-418) |
| Content And Storage | Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage |
| Species | Mammalian |
| Protein Tag | Strep-tag II, GST Tag |
| Cloning Method | Direct Cloning |
| Concentration | 250 ng/μL |
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