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Invitrogen™ Lyo-ready SuperScript™ III Flash Reverse Transcriptase
Description
Invitrogen Lyo-ready SuperScript III Flash Reverse Transcriptase joins the SuperScript product family as a reverse transcriptase designed for one-step RT-qPCR and point-of-care assay development. It is a novel SuperScript III Reverse Transcriptase variant, engineered for enhanced speed, thermostability, and inhibitor tolerance. Lyo-ready SuperScript III Flash Reverse Transcriptase provides enhanced sensitivity as low as five copies per reaction.
Benefits of Lyo-ready SuperScript III Flash Reverse Transcriptase include:
- Excellent choice for one-step RT-qPCR
- Fast cDNA synthesis (~1 minute) reduces the time to results
- Hot-start enables high-throughput applications, benchtop stability, and improved specificity of one-step RT-qPCR
- Thermostability up to 70°C allows elevated reaction temperatures and cDNA synthesis from complex RNA targets
- High inhibitor tolerance makes the system less dependent on RNA sample purity
- Commercial rights and customization enable product usage for commercial purposes
Hot start
Lyo-ready SuperScript III Flash Reverse Transcriptase is inactive at ambient temperatures due to the hot start mechanism, which enables room temperature reaction setup and improved specificity and consistency of RT-qPCR results. When used in conjunction with hot start DNA polymerase, such as Lyo-ready Platinum™ II Taq DNA Polymerase, a fully assembled master mix can be stored at room temperature for up to 24 hours, enabling high-throughput applications and compatibility with automation and point-of-care assay development. Due to enhanced specificity, this pair of enzymes also enables multiplex RT-qPCR of up to five targets.
Lyo-ready
Lyo-ready SuperScript III Flash Reverse Transcriptase is a glycerol-free enzyme that ensures compatibility with microfluidics, air-drying, and lyophilization technologies. For a full lyo-ready one-step RT-qPCR solution, we recommend using SuperScript III Flash Reverse Transcriptase together with Lyo-ready Platinum II Taq DNA polymerase. To learn more about Lyo-ready Enzymes for molecular diagnostics development visit the page thermofisher.com/lyo-ready.
OEM reverse transcriptase
To learn more about OEM and raw materials for molecular diagnostics, customization capabilities, and commercial rights, visit the page thermofisher.com/mdx.
Specifications
Specifications
| Concentration | 60 U/μL |
| Content And Storage | •Lyo-ready SuperScript™ III Flash Reverse Transcriptase, 2 kU •Store at -20°C. |
| Format | Stand-alone Enzyme |
| PCR Method | 1-step RT-qPCR |
| Reaction Speed | Fast |
| Optimal Reaction Temperature | 60°C |
| Quantity | 2 kU |
| Ribonuclease H Activity | Reduced |
| Shipping Condition | Dry Ice |
| For Use With (Application) | Virus Detection |
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For Research Use Only.
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