Invitrogen™ Lycopersicon Esculentum (Tomato) Lectin (LEL, TL), fluorescein (FITC)

Lycopersicon esculentum (Tomato) Lectin (LEL, TL), fluorescein (FITC), is a bright-green LEL-fluorophore conjugate. The tomato lectin LEL is widely used as marker of blood vessels and microglial cells in rodents and in neuroscience research. It is a valuable tool in studies of rodent tumor angiogenesis and the tracing of neovascular development in xenograft models and brain research. LEL-FITC can be applied directly to tissue sections, or it can be introduced through intravascular perfusion for fluorescence detection.

• Very bright FITC conjugate detected with traditional FITC filter set, Ex/Em: 495/515 nm
• Applications: immunofluorescence, glycobiology
• Sugar specificity: [GlcNAc] 1,3-N-acetylglucosamine
• Inhibiting/eluting sugar: chitin hydrolysate
• Blood group specificity: non-specific
• Unconjugated lectin molecular weight: 71 KDa.
• Immunofluorescence working concentration: 5–20 μg/mL

LEL is a very stable single-subunit glycoprotein containing ∼50 percent arabinose and galactose and may form multimeric aggregates in solution. It is provided at a 2 mg/mL concentration in 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM Ca²⁺. If a precipitate forms upon long-term storage, warm to 37°C.