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Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases (2 U/μL)
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Description
Includes
Phusion High-Fidelity DNA Polymerase
- Phusion DNA Polymerase 2 U/μL
- 5X Phusion HF Buffer
- 5X Phusion GC Buffer
- DMSO
- 50 mM MgCl2 solution
Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1X concentration.
Phusion Green High-Fidelity DNA Polymerase
- Phusion DNA Polymerase 2 U/μL
- 5X Phusion Green HF Buffer
- 5X Phusion Green GC Buffer
- DMSO
- 50 mM MgCl2 solution
Both Phusion Green HF Buffer and Phusion Green GC Buffer provide 1.5 mM MgCl2 in the final 1X concentration.
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is an excellent choice for cloning and other applications requiring high fidelity. Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerases.
Features of Phusion™ High-Fidelity DNA Polymerase include:
- High fidelity (52X Taq)
- Fast PCR due to short extension times (15–30 s/kb)
- Robust performance, minimal optimization needed
- High yields of PCR products with minimal enzyme amounts
- Available in Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)
Applications
- High-fidelity PCR
- Cloning
- Template generation for sequencing
- Amplification of difficult (GC-rich) templates
- Long-range PCR (up to 20 kb)
- Mutagenesis
- High throughput PCR
- Microarray
Note: Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our convenient Tm calculator.
Spezifikation
Spezifikation
| Concentration | 2 U/μL |
| Content And Storage | • Phusion DNA Polymerase, 2 U/μL • 5X Phusion Green HF and GF Buffers • DMSO • 50 mM MgCl2 Phusion Green HF and Phusion Green GC Buffers each provide 1.5 mM MgCl2 in the final 1X concentration. Store at -20°C. |
| GC-Rich PCR Performance | High |
| Polymerase | Phusion High-Fidelity DNA Polymerase |
| Reaction Speed | Fast |
| Product Type | High-Fidelity DNA Polymerase |
| Quantity | 100 units |
| Shipping Condition | Dry Ice |
| For Use With (Application) | Standard PCR, High-fidelity PCR |
| Fidelity (vs. Taq) | 52X |
| Mehr anzeigen |
For Research Use Only. Not for use in diagnostic procedures.
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