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Invitrogen™ PureLink™ HiPure Plasmid Maxiprep Kit Product Code.: 10257072
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Invitrogen™ PureLink™ HiPure Plasmid Maxiprep Kit

Product Code. 10257072
10 Preps, 10 preps
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Product Code. 10257072

Brand: Invitrogen™ K210006

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Description

The PureLink HiPure Plasmid Maxiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli.

The PureLink HiPure Plasmid Maxiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli. The Maxiprep Kit protocol will typically yield 500–850 μg plasmid DNA from 100–200 mL bacterial culture, at purity that is comparable to that achieved by two passes through cesium chloride gradients.

  • Labor and time saving- no need for extra steps to remove endotoxin and other contaminants
  • High-purity, low-endotoxin product- pure enough for mammalian cell transfection
  • Versatility—purify any type and size of plasmid DNA, including BAC, bacmids, and ssM13 DNAs

Anion-exchange chromatography for plasmid purification

  • PureLink HiPure Plasmid DNA Purification Kits use patented anion-exchange resin to purify plasmid DNA to a level equivalent to two passes through CsCl gradients
  • Resin combines excellent capacity with a fast flow rate, high resolution, high yield, and efficient endotoxin removal
  • Plasmid preparations can typically be completed in less than 2 hours

Contaminant-free DNA

  • Unlike cesium chloride gradient protocols, PureLink HiPure Plasmid Purification Systems do not use troublesome solvents to work with and to dispose of
  • Typically, plasmid DNA prepared using the PureLink HiPure Plasmid Midiprep Kit will have an A260/A280 ratio of >1.80, indicating that the DNA is reasonably free of proteins that might interfere with downstream applications
  • Endotoxin levels are typically within 0.1–1 EU/ÎĽg, making this plasmid DNA ideal for mammalian cell transfection
  • Purification time is two hours

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage • 100 mL Resuspension Buffer (R3)
• 550 μL RNase A
• 100 mL Lysis Buffer (L7)
• 100 mL Precipitation Buffer (N3)
• 300 mL Equilibration Buffer (EQ1)
• 2 × 300 mL Wash Buffer (W8)
• 200 mL Elution Buffer (E4)
• 30 mL TE Buffer
• 10 HiPure Columns
• 3 Column Holders

Store all components at room temperature.
Format Column
Isolation Technology Anion Exchange Resin
Scale Maxi
Sample Type Bacterial Culture
Elution Volume 15 mL
Final Product Type BAC DNA, Plasmid DNA
For Use With (Application) Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
High-throughput Compatibility Not High-throughput Compatible (Manual)
No. of Reactions 10 Preps
Plasmid <40kb, Low Copy Plasmid, High Copy Plasmid, BAC
Prep Scale > 200 ÎĽg (Large-Scale) Plasmid DNA
Product Line PureLink™
Product Type Plasmid MaxiPrep Kit
Quantity 10 Preps
Purity Transfection Grade
Shipping Condition Room Temperature
Target BAC DNA, Plasmid DNA
System Type PureLink™
Test Time 2 hr.
Yield 1000 ÎĽg
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Product Suggestions

Documents

Product Information

Manuals

User Guide: PureLink HiPure Plasmid DNA Purification Kits Version: MAN0000486 Rev B (11 March 2015) File size: 703.4 kB

Certificates

Certificates

A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.

5 results found
Lot Number Certificate Type Date Product Code
Lot Number2805860 Certificate TypeCertificate of Analysis Date29/01/2025 Product CodeK210006
Lot Number2819288 Certificate TypeCertificate of Analysis Date29/01/2025 Product CodeK210006
Lot Number2819292 Certificate TypeCertificate of Analysis Date29/01/2025 Product CodeK210006
Lot Number2805857 Certificate TypeCertificate of Analysis Date29/01/2025 Product CodeK210006
Lot Number2802223 Certificate TypeCertificate of Analysis Date29/01/2025 Product CodeK210006

Safety and Handling

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Product Identifier
  • PureLink HiPure Plasmid Maxiprep Kit
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
 Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
 Precautionary Statement
  • P264-Wash hands thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P332+P313-If skin irritation occurs: Get medical advice/attention.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
  • P362+P364-Take off contaminated clothing and wash it before reuse.
 Supplemental information
  • MIXTURE LIST-Contains : acetic acid

For Research Use Only. Not for use in diagnostic procedures.

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