missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Molecular Biology Reagents and Kits
  4. DNA Vectors
  5. 10113802

Invitrogen™ pRSET A, B, & C Bacterial Expression Vectors

Produktkod. 10113802
Change view
Klicka för att se tillgängliga alternativ
Quantity:
20 μg
Förpackningsstorlek:
1 set
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Produktkod. Quantity unitSize
10113802 20 μg 1 set
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Denna artikel kan inte returneras. Se returpolicy
Produktkod. 10113802 Leverantör Invitrogen™ Leverantörsnummer V35120
 Restricted Item
Estimated Shipment: 28-08-2026
Lägg i varukorgen
Lägg i varukorgen
Denna artikel kan inte returneras. Se returpolicy

Beskrivning

Innehåller

20μg of each pRSET A, B and C vector is provided lyophilized. TOP10F' E. coli stab, BL21(DE3)pLysS E. coli stab, and BL21 pRSET/lacZ control E. coli stab are also included.

Designed for high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter

  • Expression is induced by production of T7 RNA polymerase in BL21(DE3) E. coli
  • These cells also produce T7 lysozyme to reduce basal expression of target genes
  • pRSET vector offers bacteriophage T7 promoter for high-level expression
  • T7 gene 10 sequence to provide protein stability
  • N-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-HisG Antibody
  • N-terminal Xpress epitope for detection with the Anti-Xpress Antibody
  • Enterokinase cleavage site for removal of fusion tag
  • Each of three vectors provided (A, B, and C) has N-terminal tag coding sequence in a different reading frame relative to multiple cloning site to simplify in-frame cloning of the gene

Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

TRUSTED_SUSTAINABILITY

Specifikationer

Constitutive or Inducible System Inducible
Inducing Agent IPTG
Promoter T7
Product Type Bacterial Expression Vector
Selection Agent (Eukaryotic) None
Content And Storage 20 μg of each pRSET A, B, & C vector is provided lyophilized. TOP10F´, BL21(DE3)pLysS, and BL21 pRSET/lacZ control E. coli stabs are also included. Store E. coli stabs at 2–8°C. All components are guaranteed stable for 6 months when properly stored.
Antibiotic Resistance Bacterial Ampicillin (AmpR)
Cleavage EK (Enterokinase) Recognition Site
Protein Tag His Tag (6x)
Cloning Method Restriction Enzyme/MCS
Quantity 20 μg
Vector pRSET
Visa mer Visa mindre

For Research Use Only. Not for use in diagnostic procedures.

Produkttitel
Välj ett problem

Genom att klicka på Skicka bekräftar du att du kan bli kontaktad av Fisher Scientific angående feedbacken du har lämnat i detta formulär. Vi kommer inte att dela din information för andra ändamål. All kontaktinformation som tillhandahålls ska också underhållas i enlighet med vår Sekretesspolicy.