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Invitrogen™ NT-GFP Fusion TOPO™ Expression Kit
Description
Includes
GFP Fusion TOPO Expression Kits contain two boxes. The GFP Fusion TOPO TA Cloning box contains linearized and topoisomerase I-activated cloning vector, dNTPs, salt solution control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a GFP expression control plasmid. The One Shot™ box contains transformation reagents including single-use, 50μL aliquots of TOP10 Chemically Competent E. coli, S.O.C. medium, and supercoiled control plasmid.
- Provide topoisomerase-I activated pcDNA3.1/NT-GFP-TOPO vector
- Taq-amplified DNA fragments are ligated into these vectors in easy 5-minute reaction on bench top
- Vector is designed for high-level expression of transient or stable GFP fusion proteins in wide range of mammalian cells
- Expression is easily detected in living cells using fluorescence
- Recombinant proteins expressed from these vector can be detected on western blots using GFP Antiserum
Cell Analysis, Cell-Based Reporter Assays, Cellular Imaging, Constitutive Expression, Enzyme and Protein Activity Assays, Fluorescent Protein (e.g. GFP) Assays, Fluorescent Protein Assays (GFP)/Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Reporter Assays
Specifications
Specifications
| Constitutive or Inducible System | Constitutive |
| Delivery Type | Transfection |
| Promoter | CMV |
| Product Type | TOPO Expression Kit |
| Reporter Gene | GFP (Cycle 3) |
| Selection Agent (Eukaryotic) | Geneticin™ (G-418) |
| Content And Storage | GFP Fusion TOPO™ Expression Kits contain two boxes. The GFP Fusion TOPO™ TA Cloning box contains linearized and topoisomerase I-activated cloning vector, dNTPs, salt solution control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a GFP expression control plasmid. Store at -20°C. The One Shot ¤ box contains transformation reagents including single-use, 50-μl aliquots of One Shot™ TOP10 Chemically Competent E. coli, S.O.C. medium, and supercoiled control plasmid. Store the One Shot™ box at -80°C. Kits are guaranteed stable for 6 months when properly stored. |
| Protein Tag | GFP (Cycle 3) |
| Cloning Method | TOPO-TA |
| Quantity | 20 reactions |
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For Research Use Only. Not for use in diagnostic procedures.
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