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Invitrogen™ pBAD-DEST49 Gateway™ Destination Vector

Product Code. 11568696
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Quantity:
6 μg
Unit Size:
6µg
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Product Code. 11568696

Brand: Invitrogen™ 12283016

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Includes

The pBAD-DEST49 Gateway destination vector includes 6μg of vector.

Designed for rapid cloning with Gateway entry clone using lambda phage site-specific recombination and subsequent high-level, tightly regulated expression in E. coli

  • araBAD promoter for tightly regulated expression in E. coli
  • HP-thioredoxin fusion for improved protein yield and solubility
  • Enterokinase cleavage site for efficient cleavage of N-terminal fusion with EKMax™
  • C-terminal V5 and 6xHis tags for efficient detection and purification of fusion proteins
  • R sites for efficient recombination with any attL-flanked Gateway entry vector

Bacterial Expression/Cloning/Gateway Cloning/Protein Expression/Proteins, Expression, Isolation and Analysis

TRUSTED_SUSTAINABILITY

Specifications

Constitutive or Inducible System Inducible
Inducing Agent Arabinose
Promoter araBAD
Product Type Gateway System Destination Expression Vector
Selection Agent (Eukaryotic) None
Content And Storage The pBAD-DEST49 Gateway™ destination vector includes 6 μg of vector. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored.
Antibiotic Resistance Bacterial Ampicillin (AmpR)
Cleavage EK (Enterokinase) Recognition Site
Protein Tag His Tag (6x), V5 Epitope Tag, Thioredoxin
Cloning Method Gateway
Quantity 6 μg
Vector pBAD, pDEST
Product Line Gateway
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For Research Use Only. Not for use in diagnostic procedures.

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