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Invitrogen™ Dynabeads™ Untouched™ Human B Cells Kit

Use this kit to isolate pure and viable untouched B cells from PBMC by negative isolation. The kit depletes T cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes.

Brand:  Invitrogen™ 11351D

Additional Details : Weight : 0.10500kg

Product Code. 10033674

  • 10350.00 NOK / Each
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Includes: 10mL Depletion MyOne; SA Dynabeads and 2mL Antibody Mix with biotinylated monoclonal antibodies towards human CD2, CD14, CD16 (a and b), CD36, CD43 and CD235a (Glycophorin A)

Description

Description

Use this kit to isolate pure and viable untouched B cells from PBMC by negative isolation. The kit depletes T cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human B cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free B cells are truly untouched, and in perfect shape for any functional assay and application.

• High purity, recovery and viability of human untouched B cells
• Easy to use and to scale up
• No columns required

An antibody mix towards the non-B cells is added to the sample and allowed to bind to the cells. Dynabeads™ are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human B cells can be directly analyzed in a flow cytometer and used in any application.

Starting Sample:
PBMC

Order Info

Shipping Condition: Room Temperature

Specifications

Specifications

B Cells
Cell Isolation Kit
Bead
Processes ∼1 x 109 cells/kit
>97%
Research Grade
Room Temperature
Human
The Kit Contains: 10 mL Depletion MyOne™ SA Dynabeads™ and 2 mL Antibody Mix with biotinylated monoclonal antibodies towards human CD2, CD14, CD16 (a and b), CD36, CD43 and CD235a (Glycophorin A).

Store at 2°C to 8°C.
Cells
Negative Isolation
20
DYNAL™, Dynabeads™, Untouched™
MNC
∼5 x 107 cells/test
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For Research Use Only. Not for use in diagnostic procedures.