Learn More
Invitrogen™ DNase I, Amplification Grade
Description
Includes
DNase I, Amp Grade (100U), 10X DNase I Reaction Buffer, 25mM EDTA (pH 8.0)
DNase I, Amplification Grade, digests single- and double-stranded DNA to oligodexyribonuleotides containing a 5' phosphate. DNase I, Amplification Grade, is suitable for eliminating DNA during critical RNA purification procedures such as those prior to RNA-PCR amplification. Absence of RNase is tested by performing a ribonuclease assay with RNA ladder.
- Specific activity is >10,000 units/mg
- Source: Purified from bovine pancreas
- Performance and quality testing: Ribonuclease assay with RNA ladder and ability to digest single-stranded and double-stranded DNA to oligonucleotides are determined
- Unit definition: One unit increases absorbance of high molecular weight DNA solution at rate of 0.001 A260 units/min./mL of reaction mixture at 25°C
- Unit reaction conditions: 0.1M sodium acetate (pH 5.0), 5mM MgCl2, 50 μg/mL calf thymus DNA, and enzyme in 1mL for 10 min. at 25°C
Removing DNA from RNA and protein preparations, PCR & Real-Time PCR, Real Time PCR (qPCR), Reverse Transcription
Order Info
Shipping Conditions: Approved for shipment on wet or dry ice
Specifications
Specifications
| Content And Storage | Contents: 1 vial of DNase I, Amp Grade (100 U) 1 vial of 10X DNase I Reaction Buffer (1000 μL) 1 vial of 25 mM EDTA (pH 8.0) (200 μL) Store at -20°C in a non-frost-free freezer. |
| Shipping Condition | Approved for shipment on Wet or Dry Ice |
| Enzyme | DNase |
| Compatible Buffer | Reaction Buffer |
| Quantity | 100 U |
For Research Use Only. Not for use in diagnostic procedures.
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.