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Invitrogen™ DNA Polymerase I

DNA polymerase with 5'→3' and 3'→5' exodeoxyribonuclease activities

Brand:  Invitrogen™ 18010025

Additional Details : Weight : 0.00010kg

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Product Code. 10043132

  • 3975.00 NOK / Each


In stock, ready to ship: 32
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Description

Description

Performance and quality testing
Endodeoxyribonuclease assay; efficiency of DNase I-dependent nick translation determined.

Unit definition

One unit incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min at 37°C using template/primer.

Unit reaction conditions

50mM potassium phosphate (pH 7.0), 6.7mM MgCl2 , 1mM 2-mercaptoethanol, 80μg/mL template/primer, 32μM dTTP, 69nM [3H]dTTP, and enzyme in 100μL for 30 min at 37°C. Also incorporates biotinylated nucleotides.

  • Applications: DNase I-dependent nick translation, second-strand synthesis in cDNA cloning, fill-in of 5' overhangs
  • Source: purified from E. coli expressing the DNA Polymerase I gene on a plasmid

Cloning, Restriction Enzyme Cloning

Specifications

Specifications

1000 units of DNA polymerase in a storage buffer of 50 mM potassium phosphate (pH 7.0), 100 mM KCl, 1 mM DTT, and 50% (v/v) glycerol. It is supplied at 5–10 units/µl.

Store at -5°C to -30°C.
No
Wet Ice
3' - 5', 5' - 3'
DNA Polymerase I
1000 units
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For Research Use Only. Not for use in diagnostic procedures.