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Invitrogen™ DNA Polymerase I/DNase I
An optimized mixture of both enzymes for efficient nick translation of DNA
Brand: Invitrogen™ 18162016
Additional Details : Weight : 0.02000kg
Includes: DNA Polymerase I/DNase I contains 0.5 units DNA Polymerase I and 0.4 milliunits DNase I per microliter.
Description
- Application: Labeling DNA with either radiolabeled or biotinylated nucleotides
- Source: DNase I is purified from bovine pancreas; DNA Polymerase I from E. coli λ lysogen nM 964
- Performance and Quality Testing: Performance tested in nick translation reaction
- Unit Definition: One unit of DNA Polymerase I in the absence of DNase I incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template primer
- Unit Reaction Conditions: 50mM potassium phosphate (pH 7.5), 6.7mM MgCl2 , 1mM 2-mercaptoethanol, 80μg/mL template•primer, 32μM dTTP, 69nM [3H]dTTP, and enzyme in 100μL for 30 min. at 37°C
Cloning, Restriction Enzyme Cloning
Order Info
Shipping Condition: Wet Ice
Specifications
| DNA Polymerase I/DNase I contains 0.5 units DNA Polymerase I and 0.4 milliunits DNase I per microliter. Store at -20°C. | |
| DNA Polymerase I | |
| 250 U | |
| No |
| DNA Polymerase | |
| 3' - 5', 5' - 3' | |
| Wet Ice |
For Research Use Only. Not for use in diagnostic procedures.