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Invitrogen™ CIAP (Calf Intestinal Alkaline Phosphatase), 1 U/μL

Product Code. 10593263
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Quantity:
1000 U
Unit Size:
Each
Product Code. Quantity unitSize
10593263 1000 U Each
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This item is not returnable. View return policy

Product Code. 10593263

Brand: Invitrogen™ 18009027

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This item is not returnable. View return policy

Description

Phosphomonoesterase that removes 3' and 5' phosphates from DNA and RNA

  • Concentration: 20 units/μL
  • Source: Purified from calf intestinal mucosa
  • Performance and Quality Testing: Endodeoxyribonuclease, 3' exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency measured in a transformation assay
  • Unit Definition: One unit hydrolyzes 1μmol of 4-nitrophenyl phosphate in 1 min. at 37°C
  • Unit Reaction Conditions: 1M diethanolamine buffer, 10mM 4-nitrophenyl phosphate, 0.25mM MgCl2 (pH 9.8) in 900μL for 10 min. at 37°C

Dephosphorylation of 5'-phosphorylated termini of vector DNA to prevent self-ligation; Dephosphorylation of 5' termini of nucleic acids prior to forward reaction with kinase; Cloning; Restriction Enzyme Cloning

TRUSTED_SUSTAINABILITY

Specifications

Concentration 1 U/μL
Content And Storage Calf Intestinal Alkaline Phosphatase (1 unit/μL) is supplied with a vial of 10X dephosphorylation buffer [500 mM Tris-HCl (pH 8.5), 1 mM EDTA], vial of dilution buffer [50% glycerol, 25 mM Tris-HCl (pH 7.6), 1 mM MgCl2 , and 0.1 mM ZnCl2 ]. Store Calf Intestinal Alkaline Phosphatase at -20°C.
Shipping Condition Approved for shipment on Wet or Dry Ice
Enzyme CIP
Compatible Buffer Diethanolamine Buffer
Quantity 1000 U
Product Type Alkaline Phosphatase

For Research Use Only. Not for use in diagnostic procedures.

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