missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Enzymes and Inhibitors
  4. Modifying Enzymes
  5. 10747864

Invitrogen™ CIAP (Calf Intestinal Alkaline Phosphatase), 20 U/μL

Product Code. 10747864
Change view
Click to view available options
Quantity:
1000 U
Unit Size:
1ku
Product Code. Quantity unitSize
10747864 1000 U 1ku
1 options
This item is not returnable. View return policy

Product Code. 10747864

Brand: Invitrogen™ 18009019

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. Se returpolicy

Beskrivning

Phosphomonoesterase that removes 3' and 5' phosphates from DNA and RNA

  • Concentration: 20 units/μL
  • Source: Purified from calf intestinal mucosa
  • Performance and Quality Testing: Endodeoxyribonuclease, 3' exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency measured in a transformation assay
  • Unit Definition: One unit hydrolyzes 1μmol of 4-nitrophenyl phosphate in 1 min. at 37°C
  • Unit Reaction Conditions: 1M diethanolamine buffer, 10mM 4-nitrophenyl phosphate, 0.25mM MgCl2 (pH 9.8) in 900μL for 10 min. at 37°C

Dephosphorylation of 5'-phosphorylated termini of vector DNA to prevent self-ligation; Dephosphorylation of 5' termini of nucleic acids prior to forward reaction with kinase; Cloning; Restriction Enzyme Cloning

TRUSTED_SUSTAINABILITY

Specifikationer

Concentration 20 U/μL
Content And Storage Calf Intestinal Alkaline Phosphatase (20 units/μL) is supplied with a vial of 10X dephosphorylation buffer [500 mM Tris-HCl (pH 8.5), 1 mM EDTA], vial of dilution buffer [50% glycerol, 25 mM Tris-HCl (pH 7.6), 1 mM MgCl2 , and 0.1 mM ZnCl2 ]. Store Calf Intestinal Alkaline Phosphatase at -20°C.
Shipping Condition Approved for shipment on Wet or Dry Ice
Enzyme CIP
Quantity 1000 U
Product Type Alkaline Phosphatase

For Research Use Only. Not for use in diagnostic procedures.

Produkttitel
Välj ett problem

Genom att klicka på Skicka bekräftar du att du kan bli kontaktad av Fisher Scientific angående feedbacken du har lämnat i detta formulär. Vi kommer inte att dela din information för andra ändamål. All kontaktinformation som tillhandahålls ska också underhållas i enlighet med vår Sekretesspolicy.