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Invitrogen™ Calcium Phosphate Transfection Kit

Product Code. 10124462
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Quantity:
75 Reactions
Unit Size:
Each
Product Code. Quantity unitSize
10124462 75 Reactions Each
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Product Code. 10124462

Brand: Invitrogen™ K278001

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Description

Includes

2 x 12mL Tissue Culture Sterile Water, 2 x 12mL 2X HEPES Buffered Saline (HBS), 3 x 1mL 2M CaCl2, 20μg pcDNA™3.1/His/lacZ

Provides high-quality reagents to enable the introduction of DNA into eukaryotic cells via calcium phosphate co-precipitation

  • Based on forming a calcium phosphate-DNA precipitate
  • Facilitates the binding of the DNA to the cell surface. DNA then enters the cell by endocytosis
  • First developed by Graham and van der Ebb and was later modified by Wigle
  • Used to transfect a wide variety of cell types for transient expression or for producing stable transformants
  • DNA is mixed directly with a concentrated solution of CaCl2, which is then added dropwise to a phosphate buffer to form a fine precipitate
  • Aeration of the phosphate buffer while adding the DNA-CaCl2 solution helps to ensure that the precipitate that forms is as fine as possible, which is important because clumped DNA will not adhere to or enter the cell as efficiently
  • Product size: 75 reactions

Cell Culture, Plasmid Transfection, Transfection

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage 2 × 12 ml Tissue Culture Sterile Water
2 × 12 ml 2X HEPES Buffered Saline (HBS)
3 × 1 ml 2 M CaCl2
20 μg pcDNA™3.1/His/lacZ

Store all components at -5 to -30°C.
Cell Type Established Cell Lines
Format 6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample Type Plasmid DNA
Transfection Technique Chemical Transfection
For Use With (Application) Transfection
High-throughput Compatibility Not High-throughput Compatible (Manual)
Product Type Transfection Kit
Quantity 75 Reactions
Serum Compatible No

For Research Use Only. Not for use in diagnostic procedures.

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