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Invitrogen™ Bac-to-Bac™ HT Vector Kit
Designed for use as part of Bac-to-Bac Baculovirus Expression System for expression and purification of histidine-tagged recombinant proteins in Sf9, Sf21, or High Five™ Cells following bacmid generation in E. coli
Brand: Invitrogen™ 10584027
Includes: The Bac-to-Bac HT Vector Kit includes 10μg of pFastBac HT vector and pFastBac HT-CAT control vector.
Description
- Strong polyhedrin promoter for protein expression
- Three reading frames for simplified cloning
- N-terminal 6 x His tag for simple purification of recombinant fusion proteins
- TEV protease cleavage site for removal of histidine tag following protein purification
Baculovirus Production and Titering, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
| Polyhedrin | |
| The Bac-to-Bac™ HT Vector Kit includes 10 μg of pFastBac™ HT vector and pFastBac HT-CAT control vector. Store the vectors at -20°C. All components are guaranteed stable for 6 months when properly stored. |
|
| His Tag (6x) | |
| 1 kit | |
| Bac-to-Bac™ |
| Vector Kit | |
| TEV Protease Recognition Site | |
| Restriction Enzyme/MCS | |
| pFastBac |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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