IL-23 p19 Monoclonal Antibody (HNU2319), Functional Grade, eBioscience™, Invitrogen™

Description

Description: The HNU2319 monoclonal antibody is specific to the p19 subunit of human IL-23, a heterodimeric cytokine made up of two covalently linked subunits, p40 and p19. It is closely related to IL-12, with which it shares the p40 subunit. Dendritic cells and macrophages produce IL-23 in response to TLR2, TLR4, and TLR8 ligands, as well as the beta-glucan receptor Dectin-1. Human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-gamma production by naive and memory T cells, as compared to IL-12. In contrast, the mouse IL-23 biological activities are more distinctly different than those of mouse IL-12 suggesting the signaling or response elements (receptors) differ between species. The IL-23 receptor is also heterodimeric and shares the IL-12Rbeta1 chain with IL-12, while the IL-23R chain is unique to IL-23. Signaling occurs through the Jak/STAT pathway and results in RORgammat expression, which drives the differentiation of CD4^+ T-cells towards the Th17 phenotype. The monoclonal antibody HNU2319 neutralizes the bioactivity of IL-23 without affecting IL-12. Applications Reported: The monoclonal antibody HNU2319 reacts with the p19 subunit of human IL-23 and inhibits its bioactivity. Applications Tested: The ND50 of HNU2319, as determined by the neutralization of mouse IL-17A induction in balb/c splenocytes by recombit human IL-23, is 0.2-0.4 μg/mL in the presence of 2 ng/mL IL-23.

IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-γ. Mouse IL-23 does induce strong proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-γ production by naive and memory T cells, as compared to IL-12.