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IL-2 Monoclonal Antibody (JES6-5H4), eFluor™ 450, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 48-7021-80
Additional Details : Weight : 0.25000kg
Description
Description: The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine. Applications Reported: This JES6-5H4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This JES6-5H4 antibody has been tested by intracellular staining and flow cytrometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 μm post-manufacturing filtered.
Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.Specifications
IL-2 | |
Monoclonal | |
0.2 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
P04351 | |
Il2 | |
Affinity chromatography | |
RUO | |
16183 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
JES6-5H4 | |
eFluor 450 | |
Il2 | |
Interleukin-2, T-cell growth factor, TCGF, Aldesleukin | |
Rat | |
25 μg | |
Primary | |
Mouse | |
Antibody | |
IgG2b κ |
For Research Use Only.