Rat anti-Mouse IgG1, eFluor 570, clone: M1-14D12, Secondary Antibody, eBioscience™
Rat Monoclonal Secondary Antibody
Brand: Affymetrix eBioscience 41-4015-80
Additional Details : Weight : 0.25000kg
DescriptionDescription: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies.Applications Reported: This M1-14D12 antibody has been reported for use in immunohistochemical staining of frozen or formalin-fixed paraffin embedded tissue sections and immunocytochemistry.Applications Tested: This M1-14D12 antibody has been tested by immunocytochemistry after staining with a primary antibody of the mouse IgG1 isotype. This can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis.Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.Excitation: 555 nm; Emission: 570 nm
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.