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  6. Invitrogen™ Taq DNA Polymerase, native, w/W1

Invitrogen™ Taq DNA Polymerase, native, w/W1

Product Code. 10063172
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10063172 100 Unit(s) Each
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Product Code. 10063172

Brand: Invitrogen™ 18038034

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Description

Taq DNA Polymerase, native, w/W1

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The 1% solution of the detergent W-1 that is included may improve the thermostability of the enzyme when added at a final concentration of 0.05% (v/v).

Taq DNA Polymerase consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5´→3´ DNA polymerase activity and a 5´→3´ exonuclease activity (see figure). With Taq DNA Polymerase, you get:

  • Your choice of recombinant or native enzyme
  • Amplification of PCR products up to 5 kb in size
  • An enzyme that is licensed and qualified for PCR

    Applications
    Amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing

    Source
    Native enzyme is purified from Thermus aquaticus YT1. Unit definition
    One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.
    • TRUSTED_SUSTAINABILITY

    Specifications

    Concentration 5 U/μL
    Content And Storage –20°C
    Description Taq DNA Polymerase, 100 Unit, 5μL Concentration, Liquid, Colorless, 3'-A Overhang, Taq DNA Polymerase, 1X Low GC-Rich PCR Performance, Standard, Reaction Speed, Separate Components Reaction Format
    Form Liquid
    Quantity 100 Unit(s)
    For Use With (Application) Standard PCR
    Overhang 3'-A
    Includes Taq DNA polymerase (20μL), 10X PCR buffer without magnesium (1.25mL), 50mM Magnesium chloride (1mL), 1% W-1 (500μL)
    Source Thermus aquaticus YT1

    For Research Use Only. Not for use in diagnostic procedures.

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