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Invitrogen™ Silencer™ Negative Control No. 1 siRNA
Description
Silencer Negative Control No. 1 siRNA is a bioinformatically designed, non-targeting siRNA used as a negative control siRNA. It distinguishes specific effects of siRNA-mediated gene silencing from non-specific procedural effects, ensuring minimal off-target effects and providing a reliable baseline for comparison. Validated through extensive testing, it has minimal impact on gene expression, cell proliferation, viability, and morphology, making it essential for accurate results in human, mouse, and rat cells.
Negative controls, such as non-targeting siRNAs, are critical for distinguishing the specific effects of siRNA-mediated gene silencing from non-specific effects caused by the experimental procedure itself. Silencer negative control No. 1 is designed to ensure minimal off-target effects, providing a robust baseline for comparing the results of siRNA-treated samples. This negative control siRNA shares the same chemical modifications as other Silencer siRNAs.
Features of Silencer Negative Control siRNAs include:
- No significant sequence similarity to human mouse, or rat genes
- Minimal effects on cell proliferation and viability
- Matching Silencer™ siRNA chemistry
Validated through extensive testing, including microarray analysis and multi-parametric cell-based assays, Silencer Negative Control No. 1 has been proven to have minimal impact on gene expression, cell proliferation, viability, and morphology. This makes it an essential tool for researchers aiming to achieve accurate and consistent phenotypic data in human, mouse, and rat cells.
What are Silencer siRNAs?
Silencer siRNAs are designed for potency and specificity using a proprietary algorithm. These unmodified siRNAs are cost-effective siRNAs with guaranteed silencing.
Quality control
Each Silencer siRNA is synthesized and purified in state-of-the-art facilities to meet the highest quality standards. Our rigorous quality control procedures include MALDI-TOF mass spectrometry analysis and analytical HPLC to monitor purity. We also assess each annealed siRNA by gel electrophoresis to ensure proper strand annealing. The result is a premium-quality siRNA that is purified and ready for use.
Specifications
Specifications
| Content And Storage | Store at or below -20°C. Do not store in a frost-free freezer. |
| Control Type | Negative Control |
| RNAi Type | siRNA |
| Species | Human, Mouse, Rat |
| For Use With (Application) | Transfection, RNAi |
| Label or Dye | Unconjugated |
| Product Line | Silencer, Ambion |
| Product Type | siRNA |
| Purity | HPLC |
| Quantity | 5 nmol |
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Frequently Asked Questions (FAQs)
A positive control siRNA such as the Silencer Select GAPDH siRNA will demonstrate efficient transfection and help to optimize transfection conditions. A negative control siRNA is necessary to serve as a baseline for gene expression.
We offer several negative and positive controls for both Silencer and Silencer Select siRNA.
For Silencer siRNA positive control:
- Silencer Firefly Luciferase (GL2 + GL3) siRNA (Cat. No. AM4629, 5nmol)
- Silencer GAPDH siRNA (human) (Cat. No AM4605, 5nmol; AM4633, 40 nmol)
- Silencer GAPDH siRNA (human, mouse, rat) (Cat. No. AM4624, 5 nmol; AM4631, 40 nmol; AM4632, 5 x 40 nmol)
- Silencer GFP (eGFP) siRNA (Cat. No. AM4626, 5 nmol)
- Silencer KIF11 (Eg5) siRNA (human, mouse, rat) (Cat. No. AM4639, 5 nmol)
If working with Fluorescently labeled Silencer siRNA:
- Silencer Cy-3 labeled GAPDH siRNA (human, mouse, rat) (Cat. No. AM4649, 5 nmol)
- Silencer FAM-labeled GAPDH siRNA (human, mouse, rat) (Cat. NO. AM4650, 5 nmol)
If working with Silencer siRNA in vivo ready:
- Silencer GAPDH Positive Control siRNA, in vivo ready (Cat. No. 4404025, 250 nmol)
For Silencer siRNA negative controls:
- Silencer Negative Control No. 1 siRNA (Cat. Nos. AM4611, 5 nmol; AM4635, 40 nmol; AM4636, 5 x 40 nmol)
- Silencer Negative Control No. 2 siRNA (Cat. Nos. AM4613, 5 nmol; AM4637, 30 nmol)
- Silencer Negative Control No. 3 siRNA (Cat. No. AM4615, 5 nmol)
- Silencer Negative Control No. 4 siRNA (Cat. No. AM4641, 5 nmol)
- Silencer Negative Control No. 5 siRNA (Cat. No. AM4642, 5 nmol)
If working with Fluorescently labeled Silencer siRNA:
- Silencer Cy 3-labeled Negative Control No. 1 siRNA (Cat. No AM4621, 5 nmol)
- Silencer FAM-labeled Negative Control No. 1 siRNA (Cat. No. AM4620, 5 nmol)
If working with Silencer siRNA in vivo ready:
- Silencer in vivo ready (Cat. No. 4404021)
For Silencer Select siRNA Positive control:
- Silencer Select GAPDH Positive Control siRNA (Cat. No. 4390849, 5 nmol; 4390850, 40 nmol; 4404024, 250 nmol)
- Silencer Select MALAT1 Positive Control siRNA for non-coding siRNA (Cat. No. 4455877, 5 nmol)
For Silencer Select siRNA negative control:
- Silencer Select Negative Control No. 1 siRNA (Cat. No. 4390843, 5 nmol; 4390844, 40 nmol)
- Silencer Select Negative Control No. 2 siRNA (Cat. No. 4390846, 5 nmol; 4390847, 40 nmol)
If working with Silencer Select siRNA in vivo ready:
- Silencer Select Negative Control No. 1 siRNA, in vivo ready (Cat. No. 4404020, 250nmol)
For Research Use Only. Not for use in diagnostic procedures.
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