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Gibco™ Human VEGF-165 Recombinant Protein

Product Code. 10690920
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Quantity:
1 mg
10 μg
100 μg
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Code produit. 10690920

missing translation for 'mfr': Gibco™ PHC9394

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Description

Recombinant Protein

Carrier-Free Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile, distilled water or appropriate buffered solution containing 0.1% BSA to regain full activity. Make any further dilutions of the reconstituted protein in buffered solution containing a carrier protein such as PBS with 0.1% BSA. Storage: Store the lyophilized protein at 2-8°C or -20°C for long term storage, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤-20°C. Avoid repeated freeze-thaw cycles.

The vascular endothelial growth factor (VEGF) family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells.
TRUSTED_SUSTAINABILITY

Spécification

Accession Number P15692
For Use With (Application) Bioactivity
Formulation Protein with no preservative
Gene ID (Entrez) 7422
Molecular Weight (g/mol) 40 kDa
Name Human VEGF-165
Purification Method Purified
Quantity 10 μg
Storage Requirements 4°C
Regulatory Status RUO
Endotoxin Concentration <0.1 ng/μg
Gene Alias H-VEGF-165; MVCD1; M-VEGF-165; vascular endothelial growth factor 165b; vascular endothelial growth factor A; vascular endothelial growth factor A121; vascular endothelial growth factor A165; vascular permeability factor; VEGF; VEGF164; VEGF165; Vegfa; VEGF-A; VPF
Common Name VEGF-165
Gene Symbol Vegfa
Biological Activity ED50 < 6 ng/mL; determined by the dose-dependent proliferation of HUVECs.
Product Type Protein
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Sequence APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
Content And Storage 4°C
Classification Carrier-Free
Activity ED50 < 6 ng/mL; determined by the dose-dependent proliferation of HUVECs.
Bioassay Proliferation of HUVECs (Human Umbilical Vein Endothelial Cells)
Endotoxin Level <0.1 ng/μg
Shipping Condition Wet Ice
Specific Activity 1.0 x 106 - 1.7 x 105 units⁄mg
Expression System HEK293 cells
Protein Family Growth Factors & Receptors
Protein Form Recombinant, Ligand
Form Lyophilized
Protein Subtype VEGF (Vascular Endothelial Growth Factor)
Research Category Oncology, Angiogenesis, Stem Cell Research, Differentiation, Inflammation
Product Line Gibco™
Purity or Quality Grade ≥95% by SDS-PAGE and HPLC
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