Invitrogen™ Human Proteoglycans Septum Cartilage Native Protein

RP-77523 is native Proteoglycans Septum Cartilage protein purified from human septum cartilage using Guanidine-HCl extraction, pepsin digestion, ethanol precipitation, potassium-acetate extraction and chromatography. The mixture contains large proteoglycans consisting of core proteins with attached glycosaminoglycans. Major components of the mixture are aggrecan and versican, which are hyaluronic acid-binding proteoglycans. Both contain hyaluronic acid binding regions, Ig-like domains, EGF like domains and lectin-like domains. As macromolecular polyanions they expand and hydrate the ECM in cartilage, intervertebral discs, dermis, blood vessel walls and brain tissue. Aggrecan and versican are degraded by proteases of the ADAMTS family. Aggrecan is cleaved by ADAMTS 1, 4, 5, 8, 9 and 15. Versican is hydrolyzed by ADAMTS 1, 4 and 9. RP-77523 may be used as a control with corresponding anti-Proteoglycans Septum Cartilage antibodies.